Genetic diversity and phylogenetic analysis of genus Paeonia based on nuclear ribosomal DNA ITS sequence

다국어 초록 (Multilingual Abstract)

The genus Paeonia belongs to the family Paeoniaceae having significant medicinal and ornamental importance. The present investigation was undertaken with an aim to understand phylogenetic relationships of three Paeonia species (P. lactiflora, P. obovata, and P. suffruticosa) that are widely distributed in China, Korea, and Japan, using nuclear ribosomal DNA (nrDNA) internal transcribed spacer (ITS) sequence and to compare the phylogeny results with investigations reported earlier using existed sequences of the same species. The size variation obtained among sequenced nrDNA ITS region was narrow and ranged from 722 to 726 bp. The highest interspecific genetic distance (GD) was found between P. lactiflora and P. suffruticosa or P. obovata. The phylogram obtained using our nrDNA ITS sequences showed non-congruence with previous hypothesis of the phylogeny between section Paeonia and section Moutan of genus Paeonia. This result was supported by the phylogenetic relations showed in the phylogram constructed with existed sequences in NCBI. The present study suggested that P. obovata belonging to section Paeonia was phylogenetically closer to P. suffruticosa representing section Moutan of genus Paeonia than P. lactiflora belonging to section Paeonia. The main reason of the paraphyly of section Paeonia is thought to be nucleotide additivity directly caused by origin hybridization. This study provides more sequence sources of genus Paeonia, and will help for further studies in intraspecies population, and their phylogentic analysis and molecular evolution.

참고문헌 (Reference)

1 Hong DY, "Yingpingmudan, the wild relative of the king of flower, Paeonia suffruticosa Andrews" 36 : 515-520, 1998

2 He CN, "Three new oligostilbenes from the seeds of Paeonia suffruticosa" 58 : 843-847, 2010

3 Alice LA, "Phylogeny of Rubus (Rosaceae) based on nuclear ribosomal DNA internal transcribed spacer region sequences" 86 : 81-97, 1999

4 Baldwin BG, "Phylogenetic utility of the internal transcribed spacers of nuclear ribosomal DNA in plants: an example from the compositae" 1 : 3-16, 1992

5 Chang HM, "Pharmacology and Applications of Chinese Meteria Medica" World Scientific Press 165-, 1986

6 Hu SL, "Paeonol, the main active principles of Paeonia moutan, ameliorates alcoholic steatohepatitis in mice" 128 : 100-106, 2010

7 Hong DY, "Paeoniaceae, In Flora of China" Science Press and Missouri Botanic Garden Press, Beijing, China 127-132, 2001

8 Jiang S, "Inhibitory effects of Moutan cortex on immediate allergic reactions" 30 : 1707-1710, 2007

9 Hong MH, "Inhibitory Effects of Paeonia suffruticosa on Allergic Reactions by Inhibiting the NF-kappaB/IkappaB-alpha Signaling Pathway and Phosphorylation of ERK in an Animal Model and Human Mast Cells" JAPAN SOC BIOSCI BIOTECHN AGROCHEM 74 (74): 1152-1156, 2010

10 Jiang DX, "Influence of Paeonia lactiflora roots extract on cAMP-phosphodiesterase activity and related anti-inflammatory action" 137 : 914-920, 2011

11 Gao W, "In vitro culture and cultivation of Chinese medicinal plants for industrial utilization and genetic resource conservation" 3 : 116-126, 2005

12 Hong DY, "Cytogeography and taxonomy of the Paeonia obovata polyploidy complex (Paeoniaceae)" 227 : 123-136, 2001

13 Batchelor J, "Ainu economic plants" R. Meiklejohn & Co 51 : 198-240, 1893

14 Doyle JJ, "A rapid DNA isolation procedure for small quantities of fresh leaf tissue" 19 : 11-15, 1987