Study on the In-vitro Culture Method for Normal Embryonic Cell Development of Porcine Parthenogenetic Embryos

다국어 초록 (Multilingual Abstract)

In the early development of parthenogenetic embryo, cytoplasm and nucleic acid fragmentation may be a cause of lower embryo development. The purpose of this study was to evaluate whether embryonic development and apoptosis factors can be reduced by controlling the in-vitro culture environment by the addition of hormones, pregnancy serum and uterine milk. Our study showed that the activity of Casp-3 increased within the cytoplasm when artificially used hormones to induce the incubation environment, and PCNA's manifestation was low. However, the addition of pregnant serum appeared to lower the Casp-3 activity compared to the other groups. In addition, MMP-9 activity was increased and early embryo development and cytoplasmic fidelity were also increased. Therefore, the results of the present study showed that the use of gestational serum in the development of parthenogenetic embryo inhibit apoptosis and increases cytoplasmic reorganization by natural environmental control in in vitro culture.

목차 (Table of Contents)

• ABSTRACT
• INTRODUCTION
• MATERIALS AND METHODS
• Chemicals and animal ethics statement
• In vitro maturation
• Parthenogenetic activation
• In vitro culture
• Enzyme linked immunosorbent assay
• Zymography
• Immunofluorescence
• RESULTS
• Differences of embryonic cell development in each in vitro culture method
• Activity of MMPs according to embryonic cell development
• Analysis of expression and localization of genes related to cell division and apoptosis.
• DISCUSSION
• CONFLICTS OF INTEREST
• AUTHOR CONTRIBUTIONS
• AUTHOR’S POSITION AND ORCID NO.
• REFERENCES