사람 탯줄혈액으로부터 다분화능 중간엽 줄기세포의 분리 및 분화 = Isolation and Differentiation of Multi-Potent Mesenchymal Stem Cells from Human Umbilical Cord Blood

다국어 초록 (Multilingual Abstract)

Human umbilical cord blood (UCB) has been regarded as an alternative source for cell transplantation and cell therapy due to its hematopoietic potential of generating all blood cells, and mesenchymal potential of differentiating into various cells of a mesodermal origin. In this study, we pursued to isolate mesenchymal stem cells (MSCs) from UCB and to induce them to differentiate into possibly many tissue-specific cell types. During the cell culture expansion, UCB-derived mononuclear cells gave rise to adherent layers of fibroblast-like cells expressing MSCs-related antigens such as SH2, SH3, ASMA, CD13, CD29, and CD49e. Moreover, the study showed that these cells, in appropriate differentiation conditions, could differentiate, at least, into skeletal myoblasts, osteoblasts, and neuronal cells, as judged by RT-PCR, immunocytochemistry, and immunofluorescent staining against several tissue-specific markers. Our results indicate that UCB may be an alternative source of MSCs to bone marrow as UCB-derived MSCs possess a multipotency of skeletal myogenic, osteogenic, and neurogenic differentiation. And it also implies that these cells may become a suitable source for cell-based therapy as well as a useful tool of tissue engineering.

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