In vitro Induction of Tetraploid Roots by Various Pretreatments from Anther of Panax ginseng C. A. Meyer

다국어 초록 (Multilingual Abstract)

This experiment was done to determine the optimum conditions for the induction of tetraploidy in Panax ginseng C. A. Meyer using bud length, temperature and plant growth regulator pretreatments. Highest callus formation was obtained when the medium was inoculated with flower bud in the size of 2-3 mm in length. The optimum temperature for the callus formation was high when treated at 4oC for 4-5 days. Among the treatments of growth regulators and different concentration, highest callus formation was observed in combination of 5 mg/L 2,4-D and 1 mg/L kinetin for P. ginseng. As a result of flow cytometer analysis, all 7 adventitious roots were confirmed as tetraploidys. Cytological analysis revealed that the chromosome number of tetraploid roots was 96, while that of diploid roots was 48. Tetraploid ginseng roots were inoculated to flower bud size of 2-3 mm in length. The callus formation was optimum when treated with l mg/L 2,4-D at 4oC for 5 days. Compared with control roots, tetraploid roots were thicker and longer and had few lateral branches. Fresh weight of tetraploid roots was relatively higher than the control roots.

참고문헌 (Reference)

1 Dover, G. A., "The organization and polarity of pollen mother cells of Triticum aestivum" 11 : 699-711, 1972

2 Walker, R. I., "The effect of colchicine on microspore mothercells and microspores of Tradescantia paludosa" 29 : 280-285, 1938

3 Bennett, M. D., "The effect of colchicine on fibrillar material in wheat meiocytes" 38 : 33-47, 1979

4 Sugaya, A., "Proliferative effect of ginseng saponin on neurite extension of primary cultured neurons of the rat cerebral cortex" 22 : 173-181, 1998

5 Lewis, W. H., "Ploidy: Biological relevance, in: Basic Life Sciences" Plenum Press 180-182, 1980

6 Gamborg, O. L., "Nutrient requirements of suspension cultures of soybean root cells" 50 : 151-158, 1968

7 Jo, J. S., "Korean ginseng has a characteristic shape, in: Understanding of Korean Ginseng" The Society For Korean Ginseng 37-38, 1995

8 Van Tuyl, J. M., "Interspecific hybridization and polyploidization as tools in ornamental plant breeding" 312 : 13-22, 2003

9 Chen, L. L., "In vitro tetraploid induction and generation of tetraploids from mixoploids in Astragalus membranaceus" 112 : 339-344, 2007

10 Du, L. G., "In vitro pollen plant induction and embryoid clone establishment of Panax ginseng" 30 : 941-945, 1987

11 Perry, J. L., "In vitro induction of tetraploidy in Vaccinium darrowi, V. elliottii and V. darrowi × V. elliottii with colchicines treatment" 109 : 4-6, 1984

12 Gu, X. F., "In vitro induction of tetraploid plants from diploid Zizyphus jujuba Mill. cv. Zhanhua" 24 : 671-676, 2005

13 Shao, J., "In vitro induction of tetraploid in pomegranate (Punica granatum)" 75 : 241-246, 2003

14 Hu, H., "In vitro induced haploids in wheat, in: In Vitro Haploid Production in Higher Plants" Kluwer Academic Publishers 1997

15 Gosal, S. S., "Haploidy in rice, in: In Vitro Haploid Production in Higher Plants" Kluwer Academic Publishers 1-35, 1997

16 De, S. S., "Flow cytometric analysis of ploidy in Rhododendron (subgenus Tsutsusi)" 36 : 125-127, 2001

17 Arumuganathan, K., "Estimation of nuclear DNA content of plants by flow cytometry" 9 : 229-241, 1991

18 Junqiang, L., "Embryogenesis and plant regeneration from anther culture in loquat (Eriobotrya japonica L.)" 115 : 329-336, 2008

19 Rimberia, F. K., "Embryo induction via anther culture in papaya and sex analysis of the derived plantlets" 103 : 199-208, 2005

20 Palazon, J., "Elicitation of different Panax ginseng transformed root phenotypes for an improvement ginsenoside production" 41 : 1019-1025, 2003

21 Wan, Y., "Efficient production of doubled haploid plants through colchicine treat ment of anther-derived maize callus" 77 : 889-892, 1989

22 김연수, "Effect of Polyploidy Induction on Biomass and Ginsenoside Accumulations in Adventitious Roots of Ginseng" 한국식물학회 47 (47): 356-360, 2004

23 Pryor, R. L., "Colchicine-induced tetraploid azaleas" 3 : 283-286, 1968

24 Gao, S. L., "Autotetraploid plants from colchicine-treated bud culture of Salvia miltiorrhiza Bge" 47 : 73-77, 1996

25 Banerjce, U., "Antistress and antifatigue properties of Panax ginseng: Comparison with piracetan" 32 : 277-285, 1982

26 Chung, G. S., "Anther culture technology in rice, in: Rice Management Biotechnology" 1-9, 1995

27 Murthy, H. N., "Anther culture of niger" 27 : 553-558, 2000

28 Anderson, J. A., "An in vitro chromosome doubling method for clovers (Trifolium ssp.)" 34 : 1-5, 1991

29 Ishizaka, H., "Amphidiploids between Cyclamen persicum Mill. and C. hederifolim Aiton induced through colchicine treatment of ovules in vitro" 44 : 161-166, 1994

30 Jackson, B. P., "Alkaloid biogenesis in tetraploid Stramonium" 5 : 778-793, 1953

31 Murashige, T., "A revised medium for rapid growth and bioassays with tobacco tissue" 15 : 473-497, 1962

32 Griesbach, R. J., "A fertile tetraploid Anigozanthos hybrid produced by in vitro colchicine treatment" 25 : 802-803, 1990