Breeding on High Lycopene and Beta Carotene with Multi-Disease Resistance in Tomato

다국어 초록 (Multilingual Abstract)

This study was carried out to breed and develop high quality and functional nutrient tomato with multi disease resistance
as well as a stable growing adaptation for fresh market usage under protected plastic houses cultivation. The materials were used
5 inbred lines and their 6 hybrids of large tomato group, which have been bred and developed from 1999 to 2007 in Division
of Plant Resource Department of Chungnam National University. Fruit weight showed hybrid vigor effect that F1 hybrids weighed
more than their parent lines, fruit shape formed three type of oblate, deep oblate and globe shape, in firmness and pericarp
thickness have got a high significant correlation, inbred DN611 line was measured the most firm fruit with 6.04 mm pericarp
thickness. In fruit color at maturity, pink color crossed to red color appeared all red fruit color in the F1 hybrids, it means red
skin color is a dominant gene compared to pink skin color is a recessive gene in tomato, while between fruit skin color and
shoulder part color showed no any co-relationship. The sugar content and titratable acid of F1 hybrids inherited an intermediate
data of their parent lines, the flavor of KP543 inbred line and the hybrid (JB535 x KP543) revealed the better taste with high
brix and proper titratable acid content*. In beta carotene content DN611 line showed 2~3 times higher than other materials so
that its 3 hybrids contained an increased level of beta carotene, lycopene content was not so much difference among inbred lines
and F1 hybrids, of them MD508 contained higher of 8.72 mg and hybrid (JB535 x JA517) had 8.05 mg lycopene content per
100 g fruit, overall pink skin color and red skin color measured a higher lycopene content than yellow and orange skin color
at ripe stage. In disease resistance test by PCR marker for Fusarium race2 (I2), Nematode (Mi1), ToMV (Tm22), Cladosporium
(Cf9), (JB535 x JA517) hybrid have got multi-resistance with homozygote band in Nematode, ToMV, Cladosporium and heterozygote
band in Fusarium race2. Through this breeding program we could select high quality and functional nutrient with multi resistant
F1 hybrids and inbred lines in tomato which are two best hybrids (JB535 x MD508), (JB535 x JA517), additionally developed high
beta carotene inbred line DN611 and increased the level of lycopene inbred line MD508. These results will be very useful to
make a high quality tomato variety continuously.

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