서양종 꿀벌로부터 분리한 정제봉독의 통풍 억제 효능 구명

다국어 초록 (Multilingual Abstract)

Honeybee (Apis mellifera L.) venom (PBV) is a good candidate for development as a therapeutic modality. The gout is a form of inflammatory arthritis caused by excess uric acid in the bloodstream. Gout causes sudden severe joint pain. To investigate in vitro xanthine oxidase inhibitory properties of PBV for gout. PBV was studied for in vitro xanthine oxidase inhibitory activity using a spectrophotometric method and matrix metalloproteinase (MMP)-9 expression after 24 hr of monosodium urate crystal (MSU) treatment when MMP-9 production reached a maximum in RAW 264.7 cells. The highest xanthine oxidase inhibitory activity was 94.7% in the 10 μg/mL PBV. The treatment of 10 μg/mL PBV showed an inhibitory effect of MMP-9, gout inflammatory marker. PBV was evaluated the xanthine oxidase and MMP-9 inhibitory potential in our study. We suggest that PBV could be used an excellent substance for gout prevention.

참고문헌 (Reference)

1 곽충실, "한국산 고구마잎과 고구마줄기 에탄올 추출물의 in vitro 항산화, 항알레르기 및 항염증효과" 한국식품영양과학회 42 (42): 369-377, 2013

2 이양숙, "자귀나무(A. julibrissin) 수피 추출물의 생리활성" 한국식품저장유통학회 18 (18): 79-86, 2011

3 한상미, "봉독의 간이 정제 방법"

4 한상미, "봉독으로부터 Phospholipase A2 성분 분리" 한국양봉학회 32 (32): 63-67, 2017

5 서수정, "맥문동(Liriope platyphylla) 잎과 뿌리 추출물의 생리활성" 한국식품저장유통학회 17 (17): 123-130, 2010

6 Piek, T., "Venoms of the Hymenoptera" Academic Press 330-, 1986

7 Stirpe, F., "The regulation of rat liver xanthine oxidase" 244 : 3855-3863, 1969

8 Wortmann, R. L., "Recent advances in the management of gout and hyperuricemia" 17 (17): 319-324, 2005

9 Habermann, E., "On the biochemistry of bee venom peptides, melittin and apamin" 343 (343): 192-203, 1965

10 Harrold, L, "New developments in gout" 25 (25): 304-309, 2013

11 Curcio-Vonlanthen, V., "Molecular parameters in melittin immunogenicity" 3 (3): 267-276, 1997

12 Rudenko, S. V., "Modulation of melittin-induced hemolysis of erythocytes" 61 (61): 2116-2124, 1996

13 Gilman, A. G., "Goodman and Gilman’s the pharmacological basis of therapeutics" Macmillan 712-, 1985

14 Hsieh, M. S., "Expression of matrix metalloproteinase-9 (gelatinase B) in gouty arthritis and stimulation of MMP-9 by urate crystals in macrophages" 89 : 791-799, 2003

15 Sutaria, S., "Effectiveness of interventions for the treatment of acute and prevention of recurrent gout-a systematic review" 45 (45): 1422-1431, 2006

16 Dalbeth, N., "Cellular characterization of the gouty tophus: a quantitative analysis" 62 (62): 1549-1556, 2010

17 Fennell, J. F., "Antibacterial action of a bee venom fraction (melittin) against a penicillin-resistant Staphylococcus and other microorganisms" 5 : 1-13, 1967

18 Kim, H. W., "Acupoint stimulation using bee venom attenuates formalin-induced pain behavior and spinal cord fos expression in rats" 65 (65): 349-355, 2003

19 Health Insurance Review & Assessment Service, "2010 National Health Insurance Statistical Yearbook" 559-578, 2011