Recently flow cytometry has been provide a wide range of application in routine clinical use, such as DNA content, platelet antibody, reticulocyte count and chromosomal study. Among this function, immunophenotyping of lymphocyte is the principal funct...
Recently flow cytometry has been provide a wide range of application in routine clinical use, such as DNA content, platelet antibody, reticulocyte count and chromosomal study. Among this function, immunophenotyping of lymphocyte is the principal function of flow cytometry and its clinical relevance is already established for a number of disorders and is now being investigated for a variety of disorder.
The fluorescent microscopic method had been used for lymphocyte subset but it has some drawback, it is time consuming, tedious, and analyze only limited number of cells and influenced by individual's variation.
But flowcytometry rapidly analyze the individual cells in a very short time (1000 cells/sec) and can analyze in dual immunofluorescence staining in single cells. Also, only lymphocyte could be analyzed by gating technique. Forward and orthogonal light scatter and two fluorescence intensity were used to define the gate.
A comparison was made between the subsets by fluorescence microscopy and flow cytometry in which correlation coefficients of more than 0.88.
From the above results, we concluded that the flow cytometry was very useful for immunophenotyping of lymphocyte in large number of samples in a short time.