연골재생용 신규 고체과산화수소를 이용한 다공성헤파린 고정화 PLGA지지체의 제조 및 특성결정 = Preparation and Characterization of Porous Heparin-immobilized PLGA Scaffolds Using Novel Solid Hydrogen Peroxide for Cartilage Regeneration

다국어 초록 (Multilingual Abstract)

The objective of this study was to develop porous functionalized scaffold which has more biominetic and bioactivity using a heparin for cartilage regeneration. Three dimensional(3D) porous poly(D,L-lactide-co-glycolide)( PLGA) scaffold was fabricated using urea hydrogen peroxide as a novel porogen. The prepared scaffold was then functionalized through Ar plasma treatment and in situ graft polymerization of acrylic acid (AAc), and then heparin immobilization onto AAc-grafted scaffold surface. The porosity and pore size of the scaffolds were above 70% and 80-230 μm, respectively. The immobilization of heparin on the scaffold surface was confirmed from ATRFTIR analysis and toluidine blue O test. The heparin-immobilized scaffold showed stronger ability to induce chondrocyte attachment, proliferation, and viability than that of the control PLGA scaffold. The resulting porous heparinimmobilized PLGA scaffold would be more bioacive and suitable for chondrocyte culture, which will be expected to be a potentially applicable material as more biomimetic scaffolds for tissue regeneration including cartilage.