In this study, we aimed to explore the potential development of red seabream probiotics by isolating bacteria with antimicrobial activity against fish pathogens from the indigenous gut microbiota of farmed red seabream. Among the isolated strains from...
In this study, we aimed to explore the potential development of red seabream probiotics by isolating bacteria with antimicrobial activity against fish pathogens from the indigenous gut microbiota of farmed red seabream. Among the isolated strains from the indigenous gut microbiota of red seabream, 12 strains were selected as candidate probiotic bacteria based on their ability to withstand varying water temperatures, salinities, exhibit antimicrobial properties, and antibiotic resistance. These 12 strains were identified using the 16S rRNA gene sequence as follows: G1 (Bacillus licheniformis strain DSM 13), G3 (Bacillus velezensis strain CBMB205), G15 (Brevibacterium frigoritolerans strain DSM 8801), G21 (Bacillus pumilus strain ATCC 7061), J2 (Paracoccus marcusii strain MH1), J7 (Micrococcus yunnanensis strain YIM65004), R8 (Micrococcus aloeverae strain AE-6), R13 (Bacillus tequilensis strain 10b), B1 (Lactobacillus paracasei strain R094), B2 (Lactococcus lactis strain NBRC1 00933), B3 (Lactobacillus plantarum strain JCM1149), and B5 (Lactococcus lactis subsp. hordniae strain NBRC 100931). The selected 12 strains of gut microbiota exhibited significant efficacy in in vitro experiments against fish pathogenic microorganisms and demonstrated notable antibiotic susceptibility. The strains also maintained stability across experiments conducted under varying temperatures and salinities, suggesting their potential positive application in aquaculture settings.