bFGF의 서방형 방출거동을 가지는 Alginate/PCL 패치를 이용한 고막천공의 재생 = Regeneration of Tympanic Membrane Perforation Using bFGF Releasing Alginate/PCL Patch

다국어 초록 (Multilingual Abstract)

Damage of tympanic membrane (ear drum) is one of common diseases in otorhinolaryngology fields which is frequently arisen as a result of either otitis media or trauma. In recent years, basic fibroblast growth factor (bFGF) which can enhance fibroblast growth and angiogenesis has been employed into the various patches to improve restoration rate of damaged thympanic membranes. However, daily administration of growth factor has been considered as a critical limitation. In this study, we fabricated hydrophilized polycaprolactone (PCL) nanofiber patchs by electrospinning and the following oxygen plasma treatment. The patchs were impregnated and crosslinked by bFGF/alginate/CaSO4 mixture solution to provide the sustained release of bFGF. The mechanical strength and bFGF release behavior from the bFGF/alginate/PCL patches were investigated. To compare thympanic membrane regeneration through the various patches (PCL, alginate/PCL, and bFGF/alginate/PCL), in vivo animal study was also conducted using a guinea pig (acute damaged thympanic membrane model). The regeneration behavior of the thympanic membrane was evaluated by histological observations (H&E staining). From the bFGF release experiment, we observed that the bFGF is continuously released from the alginate/PCL patch, up to 700 ng during 21 days. For the animal study, the bFGF/alginate/PCL patch showed faster thympanic membrane regeneration than the other groups, probably due to the continuous release of the bFGF. From the results, we conclude that the bFGF/alginate/PCL patch can be a good candidate as an effective thympanic membrane regeneration patch.

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