Macrophomina phaseolina에 의한 콩 균핵마름병(가칭) 발생 = First Report of Charcoal Rot Caused by Macrophomina phaseolina on Glycine max in Korea

다국어 초록 (Multilingual Abstract)

Stem blight symptom of soybean was severely developed in 2016 in Hwaseong and Yeoncheon. During the seedling period, the damping-off of seedlings and the brown or black spots of cotyledons were observed. After August, the leaves began to be yellowed, and partially browned areas on leaves began to develop. After September, microsclerotia began to form even on the surface of the stems that had exhibited water-soaking symptom. After mid-October of the harvest season, the epidermis of the stem was peeled off, resulting in the formation of a large number of microsclerotia in the cortex. The pathogens isolated from these symptoms were the best in mycelial growth at 32–35°C, and the formation of microsclerotia was the most at 20–28°C. The pathogen was identified as Macrophomina phaseolina through the morphological characteristics of the pathogen and the sequencing of the internal transcribed spacer region gene. In addition, when inoculated with a soybean stem using toothpicks cultured with the pathogen, the same symptoms as seen on the soy- bean field occurred. When the pathogen was re-isolated at the lesion site, the same pathogen was isolated and identified as Macrophomina phaseolina. Based on the results, the disease is reported as soybean charcoal rot.

목차 (Table of Contents)

• 서 론 재료 및 방법 결과 및 고찰 요 약

참고문헌 (Reference)

1 Sung, J. M., "The outbreak and propagule formation of black root rot caused by Calonectria crotalariae in Korea" 19 : 228-233, 1980

2 Short, G. E., "Survival of Macrophomina phaseolina in soil and in residue of soybean" 70 : 13-17, 1980

3 Yu, S. H., "Survey on seed-borne fungi in some important crops of Korea" 9 : 275-283, 1982

4 Allen, T. W., "Soybean yield loss estimates due to diseases in the United States and Ontario, Canada, from 2010 to 2014" 18 : 19-27, 2017

5 Wrather, J. A., "Soybean disease loss estimates for the top 10 soybean producing countries in 1994" 81 : 107-110, 1997

6 Gopala, Gogoi, R., "Rapid screening technique for evaluation of maize genotypes against stalk rot complex caused by Macrophomina phaseolina and Fusarium verticilloides" 86 : 1024-1030, 2016

7 Chidambaram, P., "Production of pycnidia by Macrophomina phaseolina" 64 : 165-168, 1975

8 Yum, K. J., "Occurrence and distribution of soybean seed-borne fungi in Korea" 5 : 287-293, 1989

9 Yu, S. H., "Macrophomina phaseolina detected in seeds of Sesamum indicum and it’s pathogenicity" 19 : 135-140, 1980

10 Korean Society of Plant Pathology, "List of Plant Disease in Korea"

11 "Korea Statistical Information Service"

12 "Korea Meteorological Administration"

13 de los Santos, B., "First report of charcoal rot, caused by Macrophomina phaseolina, on blueberry in southwestern Spain" 103 : 2677-, 2019

14 Sun, S. -L., "First report of charcoal rot caused by Macrophomina phaseolina on faba bean in China" 103 : 1415-, 2019

15 Cummings, J. A., "First report of charcoal rot caused by Macrophomina phaseolina in soybean in New York" 97 : 1506-, 2013

16 Kaur, S., "Emerging phytopathogen Macrophomina phaseolina: biology, economic importance and current diagenostic trends" 38 : 136-151, 2012

17 Smith, G. S., "Compendium of Soybean Diseases" American Phytopathological Society 29-31, 1999

18 Gupta, G. K., "Biology, epidemiology and management of pathogenic fungus Macrophomina phaseolina (Tassi) Goid with special reference to charcoal rot of soybean (Glycine max (L.) Merrill)" 160 : 167-180, 2012

19 Twizeyimana, M., "A cut-stem inoculation technique to evaluate soybean for resistance to Macrophomina phaseolina" 96 : 1210-1215, 2012