<P><B>Abstract</B></P> <P>C-Phycocyanin (C-PC), a protein from green microalgae, has been suggested to possess various biological activities, including antioxidant and free radical scavenging properties. The aim of the c...
http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
https://www.riss.kr/link?id=A107463041
2018
-
SCI,SCIE,SCOPUS
학술저널
69-78(10쪽)
0
상세조회0
다운로드다국어 초록 (Multilingual Abstract)
<P><B>Abstract</B></P> <P>C-Phycocyanin (C-PC), a protein from green microalgae, has been suggested to possess various biological activities, including antioxidant and free radical scavenging properties. The aim of the c...
<P><B>Abstract</B></P> <P>C-Phycocyanin (C-PC), a protein from green microalgae, has been suggested to possess various biological activities, including antioxidant and free radical scavenging properties. The aim of the current study was to explore the effects of C-PC on the maturation of porcine oocytes and subsequent developmental competence after parthenogenetic activation and somatic cell nuclear transfer (SCNT) as well as the underlying mechanisms. There was no significant improvement in nuclear maturation rates between the control and C-PC supplementation groups (1, 3, 5, 10 μg/mL). However, supplementation of 5 μg/mL C-PC in the maturation medium significantly increased blastocyst formation and hatching rates after parthenogenetic activation (59.6 ± 3.6% and 33.0 ± 2.6% vs. 49.8 ± 3.5% and 27.4 ± 2.4%, respectively). In addition, the presence of C-PC during the maturation period significantly improved blastocyst formation rates and total cell numbers after SCNT (24.8 ± 1.9% and 42.2 ± 3.3 vs. 21.6 ± 2.2% and 39.5 ± 3.4, respectively) compared to the control group. Furthermore, cellular proliferation and the expression of pluripotency-related genes (<I>SOX2</I> and <I>NANOG</I>) were increased in cloned blastocysts derived from the C-PC supplemented group. Importantly, C-PC supplementation during maturation not only improved cumulus expansion and increased the expression of cumulus expansion-related genes (<I>HAS2</I>, <I>PTX3</I>, and <I>PTGS2</I>), but also enhanced antioxidant capacity, improved mitochondria function, and decreased cathepsin B activity in porcine oocytes. These results demonstrate that C-PC may be useful for improving porcine oocyte quality and subsequent developmental competence in embryos.</P> <P><B>Highlights</B></P> <P> <UL> <LI> C-Phycocyanin significantly increased the developmental competence of porcine parthenogenetic and cloned embryos. </LI> <LI> C-Phycocyanin enhanced antioxidant capacity, improved mitochondria function, and decreased cathepsin B activity in porcine oocytes. </LI> </UL> </P>