Polyphenol Oxidase was purified from Daucus Carota by ammonium sulfate precipitation, Sephacryl S-200 HR gel filtration and DEAE-Sephacel column chromatography.
Carrots polyphenol oxidase was purified about 31.6 folds and specific activity was 877 u...
Polyphenol Oxidase was purified from Daucus Carota by ammonium sulfate precipitation, Sephacryl S-200 HR gel filtration and DEAE-Sephacel column chromatography.
Carrots polyphenol oxidase was purified about 31.6 folds and specific activity was 877 units/mg. The enzyme has high substrate specificity on ferulic acid. The Km value for catechol was 12.5mM. Optimum pH was 5.5 and optimum temperature was 30℃. Potassium cyanide, 2-mercaptoethanol and EDTA inhibited the activity of enzyme. The activity was increased by addition of ??, ??, ??, ??, ?? and ?? ions.
The molecular weight of polyphenol oxidase was approximately 45,000 daltons.