抽出方式에 따른 防風通聖散이 脂肪細胞 代謝에 미치는 影響 = The Effect of Bangpungtongsung-san Extracts on Adipocyte Metabolism

다국어 초록 (Multilingual Abstract)

Objectives : The purpose of this study is to investigate the effects of Bangpungtongsung-san extracts on the preadipocytes proliferation, of 3T3-L1 cell line. lipolysis of adipocytes in rat's epididymis and localized fat accumulation of porcine by extraction methods(alcohol and water).
Methods : Diminish 3T3-L1 proliferation and lipogenesis do primary role to reduce obesity. So, 3T3-L1 preadipocyte and adipocytes were performed on cell cultures, and using Sprague-Dawley rats for the lipogenesis, and treated with 0.01-1㎎/㎖ Bangpungtongsung-san Extracts depend on concentrations. Porcine skin including fat tissue after treated Bangpungtongsung-san Extracts by means of the dosage dependent variation are investigated the histologic changes after injection of these extracts.
Results : Following results were obtained from the 3T3-L1 preadipocyte proliferation and lipolysis of adipocyte in rats and histologic investigation of fat tissue.
1. Bangpungtongsung-san extracts were showed the effect of decreased preadipocyte proliferation on the high dosage(1.0 ㎎/㎖).
2. Bangpungtongsung-san extracts were showed the effect of decreased the activity of glycerol-3-phosphate dehydrogenase(GPDH) on the high dosage(1.0 ㎎/㎖) and Specially, alcohol extract of Bangpungtongsung -san was clear as time goes by high concentration.
3. Bangpungtongsung-san extracts were showed tries to compare the effect of lipolysis, alcohol extract of Bangpungtongsung-san on the high dosage(1.0 ㎎/㎖) was observed the effect is higher than water extract.
4. Investigated the histological changes in porcine fat tissue after treated Bangpungtongsung-san extracts, we knew that water extract of Bangpungtongsung-san was showed the effect of lipolysis on the high dosage(10.0 ㎎/㎖) and alcohol extract of Bangpungtongsung-san was showed significant activity to the lysis of cell membranes in all concentration.
Conclusion : These results suggest that Bangpungtongsung-san extracts efficiently induces diminish proliferation of preadipocyte and lipolysis in adipose tissue.
Keywords in context: Bangpungtongsung-san, alcohol extract, Water extracts, 3T3-L1 preadipocyte, adipocytes, lipolysis, Fat tissues.

목차 (Table of Contents)

• 目次 = ⅰ
• Ⅰ. 緖論 = 1
• Ⅱ. 實驗 材料 및 方法 = 3
• 1. 材料 = 3
• 1) 防風通聖散 추출물의 조제 = 3
• 2) 3T3 지방세포 및 시약 = 3
• 3) 동물 = 4
• 4) 돼지의 지방 조직 = 4
• 2. 方法 = 6
• 1) 지방전구세포의 배양 = 6
• 2) 지방세포의 분화측정 = 6
• ① Oil Red O 염색 = 6
• ② glycerol-3-phosphate dehydrogenase(GPDH) 활성 = 6
• 3) 지방세포의 분해측정 = 7
• 4) 돼지 지방세포의 용해에 미치는 영향 = 9
• 5) 통계 분석 = 9
• Ⅲ. 結果 = 10
• 1. 지방세포의 분화에 미치는 영향 = 10
• 1) 성숙지방세포의 형성 = 10
• 2) 농도별 분화에 미치는 영향 = 12
• 3) GPDH에 미치는 영향 = 17
• 2. 지방세포의 분해(lipolysis)에 미치는 영향 = 20
• 3. 돼지 지방세포에 미치는 영향 = 23
• Ⅳ. 考察 = 26
• Ⅴ. 結論 = 31
• 參考文獻 = 32
• ABSTRACT = 36