Mechanically Reinforced Extracellular Matrix Scaffold for Application of Cartilage Tissue Engineering

다국어 초록 (Multilingual Abstract)

Scaffolds with cartilage-like environment and suitable physical properties are critical for tissue-engineered cartilage repair. In this study, decellularized porcine cartilage-derived extracellular matrix (ECM) was utilized to fabricate ECM scaffolds. Mechanically reinforced ECM scaffolds were developed by combining salt-leaching and crosslinking for cartilage repair. The developed scaffolds were investigated with respect to their physicochemical properties and their cartilage tissue formation ability. The mechanically reinforced ECM scaffold showed similar mechanical strength to that of synthetic PLGA scaffold and expressed higher levels of cartilage-specific markers compared to those expressed by the ECM scaffold prepared by simple freeze-drying. These results demonstrated that the physical properties of ECM-derived scaffolds could be influenced by fabrication method, which provides suitable environments for the growth of chondrocytes.
By extension, this study suggests a promising approach of natural biomaterials in cartilage tissue engineering.

참고문헌 (Reference)

1 신유섭, "Transplantation of Autologous Chondrocytes Seeded on a Fibrin/Hyaluronic Acid Composite Gel into Vocal Fold in Rabbits: Preliminary Results" 한국조직공학과 재생의학회 9 (9): 203-208, 2012

2 Discher DE, "Tissue cells feel and respond to the stiffness of their substrate" 310 : 1139-1143, 2005

3 Zhang Y, "The impact of PLGA scaffold orientation on in vitro cartilage regeneration" 33 : 2926-2935, 2012

4 Hu Y, "Synergistic effect of carbodiimide and dehydrothermal crosslinking on acellular dermal matrix" 55 : 221-230, 2013

5 Ing Loon Sean Chua, "Signaling of Extracellular Matrices for Tissue Regeneration and Therapeutics" 한국조직공학과 재생의학회 13 (13): 1-12, 2016

6 Wenzel RN, "Resistance of solid surfaces to wetting by water" 28 : 988-994, 1936

7 Chiang H, "Repair of articular cartilage defects: review and perspectives" 108 : 87-101, 2009

8 Young JJ, "Preparation of cross-linked hyaluronic acid film using 2-chloro-1-methylpyridinium iodide or water-soluble 1-ethyl-(3,3- dimethylaminopropyl) carbodiimide" 15 : 767-780, 2004

9 Nettles DL, "Potential use of chitosan as a cell scaffold material for cartilage tissue engineering" 8 : 1009-1016, 2002

10 Guan Y, "Porcine kidneys as a source of ECM scaffold for kidney regeneration" 56 : 451-456, 2015

11 Suhaeb A. Mahmod, "Phytoestrogen (Daidzein) Promotes Chondrogenic Phenotype of Human Chondrocytes in 2D and 3D Culture Systems" 한국조직공학과 재생의학회 14 (14): 103-112, 2017

12 Jia S, "Oriented cartilage extracellular matrix-derived scaffold for cartilage tissue engineering" 113 : 647-653, 2012

13 Haugh MG, "Novel freeze-drying methods to produce a range of collagen-glycosaminoglycan scaffolds with tailored mean pore sizes" 16 : 887-894, 2010

14 Sreejit P, "Natural ECM as biomaterial for scaffold based cardiac regeneration using adult bone marrow derived stem cells" 9 : 158-171, 2013

15 Mikos AG, "Laminated three-dimensional biodegradable foams for use in tissue engineering" 14 : 323-330, 1993

16 Liang Q, "Isolation and characterization of collagen from the cartilage of Amur sturgeon (Acipenser schrenckii)" 49 : 318-323, 2014

17 Grover CN, "Investigating the morphological, mechanical and degradation properties of scaffolds comprising collagen, gelatin and elastin for use in soft tissue engineering" 10 : 62-74, 2012

18 Athanasiou KA, "Interspecies comparisons of in situ intrinsic mechanical properties of distal femoral cartilage" 9 : 330-340, 1991

19 Choi BH, "Inhibition of blood vessel formation by a chondrocyte-derived extracellular matrix" 35 : 5711-5720, 2014

20 Luo X, "In vitro evaluation of decellularized ECMderived surgical scaffold biomaterials" 105 : 585-593, 2017

21 Bryant SJ, "Hydrogel properties influence ECM production by chondrocytes photoencapsulated in poly(ethylene glycol) hydrogels" 59 : 63-72, 2002

22 김현정, "Hybrid Scaffolds Composed of Hyaluronic Acid and Collagen for Cartilage Regeneration" 한국조직공학과 재생의학회 9 (9): 57-62, 2012

23 Liao CJ, "Fabrication of porous biodegradable polymer scaffolds using a solvent merging/particulate leaching method" 59 : 676-681, 2002

24 Tan JY, "Fabrication of channeled scaffolds with ordered array of micro-pores through microsphere leaching and indirect Rapid Prototyping technique" 15 : 83-96, 2013

25 Yang Z, "Fabrication and repair of cartilage defects with a novel acellular cartilage matrix scaffold" 16 : 865-876, 2010

26 Kong HJ, "FRET measurements of cell-traction forces and nano-scale clustering of adhesion ligands varied by substrate stiffness" 102 : 4300-4305, 2005

27 Elder BD, "Extraction techniques for the decellularization of tissue engineered articular cartilage constructs" 30 : 3749-3756, 2009

28 Ozeki M, "Evaluation of decellularized esophagus as a scaffold for cultured esophageal epithelial cells" 79 : 771-778, 2006

29 Staros JV, "Enhancement by N-hydroxysulfosuccinimide of water-soluble carbodiimide-mediated coupling reactions" 156 : 220-222, 1986

30 Chung C, "Engineering cartilage tissue" 60 : 243-262, 2008

31 이지민, "Effects of Trichostatin A on the Chondrogenesis from Human Mesenchymal Stem Cells" 한국조직공학과 재생의학회 14 (14): 403-410, 2017

32 Gilbert TW, "Decellularization of tissues and organs" 27 : 3675-3683, 2006

33 Cohen NP, "Composition and dynamics of articular cartilage: structure, function, and maintaining healthy state" 28 : 203-215, 1998

34 Athanasiou KA, "Comparative study of the intrinsic mechanical properties of the human acetabular and femoral head cartilage" 12 : 340-349, 1994

35 Fiorani A, "Comparative performance of collagen nanofibers electrospun from different solvents and stabilized by different crosslinkers" 25 : 2313-2321, 2014

36 Cheng NC, "Chondrogenic differentiation of adipose-derived adult stem cells by a porous scaffold derived from native articular cartilage extracellular matrix" 15 : 231-241, 2009

37 Griffon DJ, "Chitosan scaffolds: interconnective pore size and cartilage engineering" 2 : 313-320, 2006

38 Bacakova L, "Cell adhesion on artificial materials for tissue engineering" 53 : S35-S45, 2004

39 최경환, "Cartilage Tissue Engineering using Chondrocyte-Derived Extracellular Matrix Scaffold Suppressed Vessel Invasion During Chondrogenesis of Mesenchymal Stem Cells In Vivo" 한국조직공학과 재생의학회 9 (9): 43-50, 2012

40 Lu L, "Biodegradable polymer scaffolds for cartilage tissue engineering" 391 : S251-S270, 2001

41 허성우, "Autologous Bone Marrow Mesenchymal Cell Induced Chondrogenesis for the Treatment of Osteoarthritis of Knee" 한국조직공학과 재생의학회 13 (13): 200-209, 2016

42 Rieppo L, "Application of second derivative spectroscopy for increasing molecular specificity of Fourier transform infrared spectroscopic imaging of articular cartilage" 20 : 451-459, 2012

43 Patra D, "Antiangiogenic and anticancer molecules in cartilage" 14 : e10-, 2012

44 Chinmaya Mahapatra, "Alginate-Hyaluronic Acid-Collagen Composite Hydrogel Favorable for the Culture of Chondrocytes and Their Phenotype Maintenance" 한국조직공학과 재생의학회 13 (13): 538-546, 2016

45 Yang Q, "A cartilage ECM-derived 3-D porous acellular matrix scaffold for in vivo cartilage tissue engineering with PKH26-labeled chondrogenic bone marrow-derived mesenchymal stem cells" 29 : 2378-2387, 2008

46 Moroni L, "3D fiber-deposited scaffolds for tissue engineering: influence of pores geometry and architecture on dynamic mechanical properties" 27 : 974-985, 2006