유전체재해석(resequencing)에 의한 가야벼의 벼멸구 저항성 유전자 탐색 및 선발마커 개발 = Detection of Genes Conferring Resistance to the Brown Planthopper (BPH) in Gayabyeo Through Genome Resequencing and Development of Their Selection Markers

다국어 초록 (Multilingual Abstract)

Gayabyeo, a Tongil-type rice variety, has been known to be resistant to the brown planthopper (BPH) in Korea. For genetic analysis of BPH resistance of Gayabyeo, we developed an F2 and F3 population derived from a cross between Gayabyeo and Taebaegbyeo which is a Tongil-type BPH susceptible rice variety. Based on the previously detected 284,501 putative SNPs between Gayabyeo and Taebaegbyeo, 99 cleaved amplified polymorphic sequences (CAPS) markers were developed, and they have been used for genotyping 180 F2 plants. By comparison of resequencing data of Gayabyeo and the sequences of already reported BPH resistance genes (Bph3, BPH9, Bph14, BPH18, BPH26), it was revealed that Gayabyeo has Bph3 and BPH26 resistance genes. Two InDel markers, Bph3IND and BPH26IND, were developed, which can be used as selection markers in breeding program aiming at introducing BPH resistance genes of Gayabyeo into Korean high quality japonica rice varieties. In addition, BPH bioassay was performed with 180 F3 lines for BPH resistance QTL analysis. Two major QTLs were found on chromosome 4 and 12. The regions of these two QTLs included Bph3 and BPH26, which also supported that Gayabyeo has Bph3 and BPH26 resistance genes. These results would be useful in accelerating development of various BPH-resistant high quality japonica rice varieties in Korea.

참고문헌 (Reference)

1 지현소, "유전체재해석(resequencing)에 의한 통일형 벼 품종간 단일염기서열변이(SNP) 탐색" 한국육종학회 48 (48): 460-469, 2016

2 Kim YH, "Varietal resistance of Korea rice cultivars to brown planthopper and whitebacked planthopper and their resistance sources" 31 : 7-13, 1989

3 Sogawa K, "The rice brown planthopper: feeding physiology and host plant interactions" 27 : 49-73, 1982

4 Lee JO, "Study on brown planthopper biotypes. 3. Plant damage of different rice cultivars to combination of biotypes" 28 : 52-55, 1986

5 Lee JO, "Study on brown planthopper biotypes. 2. Resistance mechanism of different rice varieties to Korean brown planthopper biotypes" 27 : 73-78, 1985

6 Fujita D, "Rice Resistance to Planthoppers and Leafhoppers" 32 : 162-191, 2013

7 Kim DH, "Resistance aspects on different growth stages and field resistance of rice varieties to brown planthopper, Nilaparvata lugens STAL (Homoptera : Delphacidae)" 33 : 33-39, 1991

8 Uhm KB, "Relationship between some weather conditions and immigration of the brown planthopper, Nilaparvata lugens Stal" 27 : 200-210, 1988

9 Hu J, "Recent progress on the genetics and molecular breeding of brown planthopper resistance in rice" 9 : 30-, 2016

10 Basten CJ, "QTL Cartographer: A reference manual and tutorial for QTL mapping" 1996

11 Hu J, "Pyramiding and evaluation of three dominant brown planthopper resistance genes in the elite indica rice 9311 and its hybrids" 69 : 802-808, 2013

12 Park YD, "Preference, development and fecundity of the brown planthopper (Nilaparvata lugens Stal) biotypes fed on different cultivars of rice with various resistance gene" 27 : 87-93, 1988

13 Cantindig JLA, "Planthoppers: new threats to the sustainability of intensive rice production systems in Asia" International Rice Research Institute 191-220, 2009

14 Seo BY, "Planthoppers: new threats to the sustainability of intensive rice production systems in Asia" International Rice Research Institute 303-314, 2009

15 Otuka A, "Planthoppers: new threats to the sustainability of intensive rice production systems in Asia" International Rice Research Institute 221-230, 2009

16 Myint KK, "Mapping and pyramiding of two major genes for resistance to the brown planthopper (Nilaparvata lugens [Stal]) in the rice cultivar ADR52" 124 : 495-504, 2012

17 Lorieux M, "MapDisto: fast and efficient computation of genetic linkage maps" 30 : 1231-1235, 2012

18 Ji H, "Map-based cloning and characterization of the BPH18 gene from wild rice conferring resistance to brown planthopper (BPH) insect pest" 6 : 34376-, 2016

19 Tamura Y, "Map-based cloning and characterization of a brown planthopper resistance gene BPH26 from Oryza sativa L. ssp. indica cultivar ADR52" 4 : 5872-, 2014

20 Huang Z, "Identification and mapping of two brown planthopper resistance genes in rice" 102 : 929-934, 2001

21 Du B, "Identification and characterization of Bph14, a gene conferring resistance to brown planthopper in rice" 106 : 22163-22168, 2009

22 Wilson MR, "Handbook for the identification of leafhoppers and planthoppers of rice" 1991

23 Bo Yoon Seo, "Electrical penetration graphic waveforms in relation to the actual positions of the stylet tips of Nilaparvata lugens in rice tissue" 한국응용곤충학회 12 (12): 89-95, 2009

24 Qiu Y, "Development and characterization of japonica rice lines carrying the brown planthopper-resistance genes BPH12 and BPH6" 124 : 485-494, 2012

25 Hibino H, "Biology and epidemiology of rice viruses" 34 : 249-274, 1996

26 Zhao Y, "Allelic diversity in an NLR gene BPH9 enables rice to combat planthopper variation" 113 : 12850-12855, 2016

27 Liu Y, "A gene cluster encoding lectin receptor kinases confers broad-spectrum and durable insect resistance in rice" 33 : 301-305, 2014