Analysis of in vitro digestion using human gut microbiota in adult and elderly individuals

다국어 초록 (Multilingual Abstract)

In vitro human digestion models are widely used to determine the digestibility of bioactive substances and to perform drug delivery analyses. To develop the most accurate in vitro human digestion model reported to date, we simulated all digestion conditions, including pH and digestion time, with changes in the amount of digestive enzymes, motility, and proportion of human gut microbiota in adult and elderly individuals. Using this newly developed model, the digestibility of bioactive materials between adult and elderly individuals. This was demonstrated through an experiment comparing the digestibility of different types of emulsified vitamin E between adult and elderly individuals. The digestibility of vitamin E emulsified by lard was found to be significantly different between adults and the elderly. Therefore, it can be concluded that this protocol provides a novel method for measuring in vitro human digestion to compare differences in drug delivery between adult and elderly individuals. In addition, this study can help to improve our understanding of the in vitro human digestion system for adults and the elderly.