Influence of Genistein Isoflavone on Matrix Metalloproteinase-2 Expression in Prostate Cancer Cells

다국어 초록 (Multilingual Abstract)

We investigated the expression of matrix metalloproteinase (MMP)-2 in human LNCaP and PC3 prostate can-cer cell lines in response to genistein exposure. Initially we studied the phytosensitivity of the cells to genistein using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay to determine percentage cell viability/inhibition and the ter-minal deoxynucleotidyl transferase-mediated fluorescein-dUTP nick end-labeling apoptosis assay to assess the type of celldeath. The results revealed that genistein inhibited growth and proliferation in both PC3 (hormone-dependent) and LNCaP(hormone-independent) prostate cancer cell lines, that there was no significant difference in sensitivity to genistein betweenPC3 and LNCaP cells, and that the effect of genistein on the cells was dose- and time-dependent. The results also revealedthat inhibition of cell growth in both PC3 and LNCaP cells was predominantly due to apoptotic cell death. These results wereconsistent with data in previous studies. This was followed by determination of the MMP-2 profile in response to genisteintreatment. The results indicated a significant dose- and time-dependent inhibition of MMP-2 expression levels in both cells,with a highly significant negative correlation between MMP-2 levels and concentration of genistein. This is of phytothera-peutic significance in view of the pivotal role of MMP-2 expression in the pathogenesis of prostate cancer. Increasing ex-pression of MMPs has been identified in many human cancers, including prostate cancer. Our findings indicate that genisteincould be a potent therapeutic inhibitor of MMP-2 in line with current concepts of targeted treatment.

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