Acer okamotoanum Inhibit the Hydrogen Peroxide-Induced Oxidative Stress in C6 Glial Cells

다국어 초록 (Multilingual Abstract)

Chronic oxidative stress due to the accumulation of reactive oxygen species (ROS) in neuronal cells ultimately leads to neurodegenerative diseases. The use of natural therapies for the prevention of ROS-induced cell damage and for the treatment of neurodegenerative disorders has shown promising results. In this study, we evaluated the neuroprotective effects of the ethyl acetate (EtOAc) fraction of A. okamotoanum against the hydrogen peroxide (H2O2)-induced oxidative stress in C6 glial cells. Results show that cell viability was decreased in cells incubated with H2O2, whereas the addition of EtOAc fraction treatments in such cells significantly increased viability. The EtOAc fraction showed the highest inhibitory activity against ROS production and it also decreased the expressions of inflammatory proteins including cyclooxygenase-2, inducible nitric oxide synthase and interleukin-1b. Furthermore, the EtOAc fraction inhibited apoptosis by regulating the protein expressions cleaved caspase -9, -3, poly ADP ribose polymerase, Bax and Bcl-2. Therefore, these results show that the EtOAc fraction of A. Okamotoanum exhibits neuroprotective effects against H2O2 induced oxidative damage by regulating the inflammatory reaction and apoptotic pathway.

참고문헌 (Reference)

1 Heo, S. J., 20 : 1087-1095, 2008

2 Kwon, S. H., 142 : 337-345, 2012

3 Bhat, A. H., 74 : 101-110, 2015

4 Qin, B., 102 : 72-79, 2014

5 Kim, H. J., 61 : 145-148, 1998

6 Ko, S. C., 50 : 2294-2302, 2012

7 Ly, J. D., 8 : 115-128, 2003

8 Kitamura, Y., 780 : 260-269, 1998

9 Alamdary, S. Z., 64 : 403-419, 2012

10 Shi, X., 194 : 63-70, 1999

11 Pahl, H. L., 18 : 6853-6866, 1999

12 Quincozes-Santos, A., 8 : e64372-, 2013

13 Mosmann, T., 65 : 55-63, 1983

14 Ma, W. W., 38 : 1315-1323, 2013

15 Park, J. H., 6 : 21857-, 2016

16 Park, H. R., 15 : 171-181, 2015

17 Kwon, S. H., 80 : 41-51, 2015

18 Budzynska, B., 232 : 931-942, 2015

19 Barnham, K. J., 3 : 205-214, 2004

20 Malek, R., 59 : 25-33, 2007

21 Janssen-Heininger, Y. M., 28 : 1317-1327, 2000

22 Moneada, S., 43 : 109-142, 1991

23 Suh, N., 58 : 717-723, 1998

24 DuBois, R. N., 110 : 1259-1262, 1996

25 Ogura, M., 58 : 504-511, 2011

26 최수연, "우산고로쇠의 항산화 및 신경세포에서의 산화적 스트레스 개선 효과" 한국응용생명화학회 60 (60): 141-147, 2017

27 정명훈, "고로쇠와 우산고로쇠 저온고압 수용성 추출물의 유용생리활성 비교" 한국약용작물학회 17 (17): 407-414, 2009

28 김영, "고로쇠와 우산고로쇠 나무의 항산화능 및 glutathione S-transferase 활성 비교" 한국약용작물학회 16 (16): 427-433, 2008

29 Syed Abdul Qadir, "고로쇠와 우산고로쇠 나무의 부위별 항암 및 면역조절능 비교" 한국약용작물학회 15 (15): 405-410, 2007

30 정명훈, "고로쇠 및 우산고로쇠나무의 부위별 미백 및 피부면역 활성" 한국산림과학회 99 (99): 470-478, 2010

31 Wen Yi Jin, "Antioxidant Activity of Cleomiscosins A and C Isolated from Acer okamotoanum" 대한약학회 30 (30): 275-281, 2007

32 최수연, "Acer okamotoanum improves cognition and memory function in Ab25–35-induced Alzheimer’s mice model" 한국응용생명화학회 60 (60): 1-9, 2017