Diagnosis of Cylindrocarpon destructans Using Enzyme-Linked Immunosorbent Assay

다국어 초록 (Multilingual Abstract)

Cylindrocarpon destructans causes ginseng root rot and produces radicicol that has an antifungal effect. In this study, we developed a method to detect this fungus using enzyme-linked immunosorbent assay (ELISA). Secreted proteins of C. destructans were used as antigens to obtain C. destructans-specific IgG from mouse. Out of 318 monoclonal antibodies generated from mouse, two antibodies (Cd7-2-2 and Cd7-2-10) showed highest specificity and sensitivity. Indirect ELISA using both antigens successfully detected C. destructans in soils, but direct ELISA using IgG conjugated with horseradish peroxidase failed to detect antigens in soils. The indirect ELISA developed here can efficiently detect the fungus and help manage ginseng root rot disease in fields.

참고문헌 (Reference)

1 신지훈, "인삼 뿌리썩음병 발병에 미치는 토양전염성병원균과 토양환경요인" 한국토양비료학회 45 (45): 370-376, 2012

2 강원명, "꿀벌 미국부저병 진단 Kit 개발" 한국양봉학회 25 (25): 105-114, 2010

3 Méchin, V., "Total protein extraction with TCA-acetone" 355 : 1-8, 2007

4 Leslie, J. F, "The Fusarium Laboratory Manual" Blackwell Publishing 388-, 2006

5 Chung, H. S, "Studies on Cylindrocarpon destructans (Zins.)Scholten causing root rot of ginseng" 12 : 127-138, 1975

6 Chevrier, M. -C., "Sensitive detection of human IgG in ELISA using a monoclonal anti-IgG-peroxidase conjugate" 23 : 362-367, 2004

7 도은수, "RT-PCR KIT의 개발" 한국자원식물학회 16 (16): 32-37, 2003

8 Neema Kassim, "Production of Mouse Anti-Quail IgY and Subsequent Labeling with Horseradish Peroxidase Using Cyanuric Chloride" 한국미생물·생명공학회 23 (23): 527-533, 2013

9 Reeleder, R. D, "Pathogenicity of Pythium species, Cylindrocarpon destructans, and Rhizoctonia solani to ginseng seedlings in Ontario" 16 : 311-316, 1994

10 장창순, "Nested PCR 기법을 이용한 인삼 뿌리썩음병원균의 특이적 검출" 한국식물병리학회 11 (11): 48-55, 2005

11 Lin, A. V, "Indirect ELISA" 1318 : 51-59, 2015

12 강윤희, "Development of a Selective Medium for the Fungal Pathogen Cylindrocarpon destructans Using Radicicol" 한국식물병리학회 30 (30): 432-436, 2014

13 Ming, F., "Development of a DAS-ELISA for detection of H9N2 avian influenza virus" 263 : 38-43, 2019

14 FARH MOHAMED ELAGAMY AHMED AHMED, "Cylindrocarpon destructans/Ilyonectria radicicola-species complex: Causative agent of ginseng root-rot disease and rusty symptoms" 고려인삼학회 42 (42): 9-15, 2018

15 Yunhee Kang, "Chlamydospore Induction from Conidia of Cylindrocarpon destructans Isolated from Ginseng in Korea" 한국균학회 44 (44): 63-65, 2016