The purpose of this paper was to study the antibacterial effect of the inhibitory system against bacterial flora in raw milk that may possibly be exploited to increase the keeping quality and to reduce initial flora.
The antibacterial effect against ...
The purpose of this paper was to study the antibacterial effect of the inhibitory system against bacterial flora in raw milk that may possibly be exploited to increase the keeping quality and to reduce initial flora.
The antibacterial effect against Ps. fluorescens KFCC-32394 in a semi-synthetic medium were not obtained with one or two of components among lactoperoxidase, thiocyanate and hydrogen peroxide, but it could be obtained by adding all of 3 components. In a semisynthetic medium containing 0.25mM thiocyanate and equimolar amount of hydrogen peroxide the bactericidal activity against Ps. fluorescens were obtained a similar efficient within 1.0~4.0 μg/lml of lactoperoxidase.
And Ps. fluorescens in a semi-synthetic medium containing 1μg/ml of lactoperoxidase was substantially reduced more than 95% at equimolar concentration of thiocyanate and hydrogen peroxide between 0.2~0. 4mM.
In raw milk by adding 0.25mM hydrogen peroxide the bacterial number were reduced to about 50%, and the raw milk could be stored for two days at 7℃. And by adding 0.25mM hydrogen peroxide with same amount of thiocyanate, 95% of the total microflora could be inhibited upto 2 days at 7℃, but stored without spoilage for 4 days at 7℃.