Clinical Significance of Monitoring Circulating CD4+CD25+ Regulatory T Cells in Kidney Transplantation during the Early Posttransplant Period

다국어 초록 (Multilingual Abstract)

The CD4+CD25+ T regulatory cells (Tregs) play an important role in immune tolerance

in experimental transplantation but the clinical significance of circulating Tregs

in the peripheral blood is undetermined. In 50 kidney transplant (KT) recipients, 29

healthy controls and 32 liver transplant (LT) recipients, the frequency of Tregs was

measured with flow cytometry before and after transplantation. In the KT recipients,

IL-10 secretion was measured with an enzyme-linked immunospot (ELISPOT) assay.

The median frequency of circulating Tregs before KT was similar to that in healthy

controls but significantly lower than that in LT patients before transplantation. The

frequency of Tregs was significantly decreased in patients with subclinical acute

rejection compared with those without subclinical acute rejection. Calcineurin inhibitors

(CNIs) and anti-CD25 antibody decreased the frequency of Tregs but mTOR inhibitor

did not. The frequency of donor-specific IL-10 secreting cells did not correlate with

the number of Tregs. The frequency of circulating Tregs in KT recipients is strongly

affected by CNIs and anti-CD25 antibody, and a low frequency of Tregs is associated

with subclinical acute rejection during the early posttransplant period.

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