차나무 겹둥근무늬병 방제용 미생물제제 개발을 위한 길항세균Bacillus subtilis BD0310의 대량배양 최적조건 = Optimum Cultivation Conditions for Mass Production of an Antagonistic Bacterium Bacillus subtilis BD0310 for Development of a Microbial Agent Controlling Gray Blight of Tea Plants

다국어 초록 (Multilingual Abstract)

Bacillus subtilis BD0310 isolated from tea leaves was used for the development of a biofungicide against Pestalotiopsis
longiseta causing gray blight of tea plants. The optimum growth conditions were investigated for the
mass cultivation of the microbial agent. The optimum temperature and cultivation time were determined as
12~24 hours at 30oC and the optimum initial pH was pH 7.0 in nutrient broth. Among the tested carbon
sources of fructose, galactose, glucose, glycerol, inositol, lactose, maltose, sorbitol and starch, maltose and
inositol were found to highly increase antifungal activity of the microbial agent against P. longiseta. Yeast
extract and tryptone apparently increased antifungal activity of the microbial agent among the tested nitrogen
sources of casein, tryptone, malt extract, yeast extract and (NH4)2SO4. The results will make a contribution
to mass production of the antagonistic bacterium Bacillus subtilis BD0310 for development of a microbial
agent controlling gray blight of tea plants.

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