<P>Herein, we report a homogeneous assay for protein kinase activity using an electrochemistry-based probe. The approach involves a peptide substrate conjugated with a redox tag and the phosphate-specific receptor immobilized on an electrode sur...
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https://www.riss.kr/link?id=A107707177
2014
-
SCOPUS,SCIE
학술저널
10992-10995(4쪽)
0
상세조회0
다운로드다국어 초록 (Multilingual Abstract)
<P>Herein, we report a homogeneous assay for protein kinase activity using an electrochemistry-based probe. The approach involves a peptide substrate conjugated with a redox tag and the phosphate-specific receptor immobilized on an electrode sur...
<P>Herein, we report a homogeneous assay for protein kinase activity using an electrochemistry-based probe. The approach involves a peptide substrate conjugated with a redox tag and the phosphate-specific receptor immobilized on an electrode surface. The peptide substrate phosphorylated by a protein kinase binds to the receptor site of the probe, which results in a redox current under voltammetric measurement. Our method was successfully applied even in the presence of citrated human blood and modified to enable a single-use, chip-based electrochemical assay for kinase activity.</P><P><B>Graphic Abstract</B>
<IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancham/2014/ancham.2014.86.issue-22/ac502549s/production/images/medium/ac-2014-02549s_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ac502549s'>ACS Electronic Supporting Info</A></P>