Expansion of human mesenchymal stem cells using the wave bioreactor system with pulsed electromagnetic field stimulation for enhanced cell culture performance = Expansion of human mesenchymal stem cells using the wave bioreactor system with pulsed electromagnetic field stimulation for enhanced cell culture performance

다국어 초록 (Multilingual Abstract)

Most bone tissue-engineering models fail to demonstrate the complex cellular functions of living bone; therefore, most translational studies on bone tissue are performed in live models. To reduce the need for live models, we developed a stimulated micro-chip model for monitoring protein secretion during osteogenesis using human mesenchymal stem cells (hBMSCs). We established a bone micro-chip system for monitoring the in vitro differentiation and sensing the secreted proteins of hMSCs under a sinusoidal electromagnetic field (SEMF), which ameliorates bone healing in a biomimetic natural bone matrix. A 3V-1Hz SEMF biophysically stimulated osteogenesis by activating ERK-1/2 and promote phosphorylation of p38 MAPK kinases. Exposure to a 3V-1Hz SEMF upregulated the expression of osteogenesis-related genes, and enhanced the expression of key osteoregulatory proteins. We identified 23 proteins that were differentially expressed in stimulated hBMSC secretomes, or were absent in the control groups. Our on-chip stimulation technology is easy to use, versatile, and non-disruptive, and should have diverse applications in regenerative medicine and cell-based therapies.