<P>Abstract</P><P>Aims: </P><P>To fabricate a DNA chip containing random fragments of genomic DNA of <I>Yersinia enterocolitica</I> and to verify its diagnostic ability.</P><P>Methods and Resu...
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https://www.riss.kr/link?id=A107587357
2010
-
SCOPUS,SCIE
학술저널
665-670(6쪽)
0
상세조회0
다운로드다국어 초록 (Multilingual Abstract)
<P>Abstract</P><P>Aims: </P><P>To fabricate a DNA chip containing random fragments of genomic DNA of <I>Yersinia enterocolitica</I> and to verify its diagnostic ability.</P><P>Methods and Resu...
<P>Abstract</P><P>Aims: </P><P>To fabricate a DNA chip containing random fragments of genomic DNA of <I>Yersinia enterocolitica</I> and to verify its diagnostic ability.</P><P>Methods and Results: </P><P>A DNA microarray chip was fabricated using randomly fragmented DNA of <I>Y. enterocolitica</I>. Chips were hybridized with genomic DNA extracted from other <I>Y. enterocolitica</I> strains, other <I>Yersinia</I> spp. and bacteria in different genera. Genomic DNA extracted from <I>Y. enterocolitica</I> showed a significantly higher hybridization rate compared with DNA of other <I>Yersinia</I> spp. or bacterial genera, thereby distinguishing it from other bacteria.</P><P>Conclusions: </P><P>A DNA chip containing randomly fragmented genomic DNA from <I>Y. enterocolitica</I> can detect <I>Y. enterocolitica</I> and clearly distinguish it from other <I>Yersinia</I> spp. and bacteria in different genera.</P><P>Significance and Impact of the Study: </P><P>A microarray chip containing randomly fragmented genomic DNA of <I>Y. enterocolitica</I> was fabricated without sequence information, and its diagnostic ability to identify <I>Y. enterocolitica</I> was verified.</P>