Isolation and Identification of Fusicoccum speciesfrom Quercus dentata

다국어 초록 (Multilingual Abstract)

An imperfect fungus Fusioccum species was isolated from Quercus dentata. A naturallyinfected Daimyo oak tree was collected and showed elongate wounds on the stem. The fungal cultures wereinitially white and cottony, and later turned dark gray. Numerous solitary pycnidia were developed on theculture plates. Conidial mases were swollen and measured as aproximately 100 to 30 m in length. Itappeared that conidia were usualy held together in globose to oval drops. Conidia were hyaline, single-celed (nonseptate), ellipsoid to fusoid, and measured as approximately 8.0. 2.7 m. Based on thesecultural and morphological characteristics, the fungal isolate was identified as a species of FusicoccumCorda. To preserve and examine fungal spores exuded from pycnidia on the medium surface, a vaporexposed to the vapor of 2% (v/v) glutaraldehyde and 2% (w/v) osmium tetroxide each for 2 h. With thevapor fixation we obtained excellent retention of conidial masses in this study. The simple and versatileprocedure for demonstrating fungal spores and their exudation from fruiting bodies would facilitatecharacterization of diverse pathological and environmental isolates as they are in native environments.

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