염색배제 분석법에 의한 HEp-2 세포주의 항암제 감수성 시험에 관한 실험적 연구 = Chemosensitvity of HEp-2 cell line to anticancer drugs using dye exclusion assay

다국어 초록 (Multilingual Abstract)

The in vitro predictive test of tumor sensitivity to antineoplastic drugs analogous to the antibiotics sensitivity test has been a high priority of cancer research. Cell culture provides a rapid and efficient system for chemosensitivity of anticancer drugs. Cancer cell line from human (HEp-2) was treated with drugs under tissue culture condition. Cell survival of HEp-2 cells after exposure with anticancer drugs(10^5 viable cells / drug for 1 hr) was assessed by dye exclusion assay 〔DEA〕. All tests were done in triplicate.
The obtained results were as follows :
1. 〔DEA〕 results demonstrated the significance of cytotoxicity in determining sensitivity to anticancer drugs at standard concentration ; CDDP, 5-FU(p<0.05), ADR, BLM, MTX, MMC, VBL(p<0.01).
2. HEp-2 cells were resistive to 5-FU, MMC, at low concentration, sensitive to VBL at all concentration.
3. The difference of among 7 drugs was observed in 〔DEA〕. HEp-2 cells were sensitive to VBL, ADR(p<0.01) at all concentrations, and MMC at standard and high concentrations (p<0.05).