Sulfuretin Inhibits Ultraviolet B-induced MMP Expression in Human Dermal Fibroblasts

다국어 초록 (Multilingual Abstract)

Sulfuretin is one of the main flavonoids produced by Rhusverniciflua. Sulfuretin has been shown to exhibit many pharmacological activities including anti-oxidant, anti-obesity, anti-inflammatory and anti-mutagenic activities. However, the anti-skin photoaging effects of sulfuretin has not yet been reported. In the present study, we investigated the inhibitory effect of sulfuretin on the expression levels of MMP-1 and -3 in the human dermal fibroblast cells. Western blot analysis and real-time PCR revealed sulfuretin inhibited UVB-induced MMP-1 and -3 expressions in a dose-dependent manner. UVB-induced MAPK/NF-κB/p50 activation and MMP expression were completely blocked by pretreatment of sulfuretin. Taken together, sulfuretin could prevent UVB-induced MMP expressions through inhibition of MAPK/NF-κB/p50 activation.

참고문헌 (Reference)

1 Thanos, D., "a lesson in family values" 80 : 529-532, 1995

2 Brenneisen, P., "Ultraviolet-B irradi-ation and matrix metalloproteinases: from induction via signaling to initial events" 973 : 31-43, 2002

3 Chung, J.H., "Ultraviolet modulation of human macrophage metalloelastase in human skin in vivo" 119 : 507-512, 2002

4 Rittie, L, "UV-light-induced signal cascades and skin aging" 1 : 705-720, 2002

5 Oh, J.H., "Thioredoxin secreted upon ultraviolet A irradiation modulates activities of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 in human dermal fibroblasts" 423 : 218-226, 2004

6 Pasparakis, M., "TNF-mediated inflammatory skin disease in mice with epidermis-specific deletion of IKK2" 417 : 861-866, 2002

7 Lee, S.O., "Suppression of PMA-induced tumor cell invasion by capillarisin via the inhibition of NF-kappaB-dependent MMP-9 expression" 366 : 1019-1024, 2008

8 Mi-Young Song, "Sulfuretin protects against cytokine-induced β-cell damage and prevents streptozotocin-induced diabetes" 생화학분자생물학회 42 (42): 628-638, 2010

9 Lee, S.O., "Silibinin suppresses PMA-induced MMP-9 expression by blocking the AP-1 activation via MAPK signaling pathways in MCF-7 human breast carcinoma cells" ACADEMIC PRESS INC ELSEVIER SCIENCE 354 : 165-165~171, 2007

10 Jackson, C, "Selective matrix metalloproteinase (MMP) inhibition in rheumatoid arthritis--targetting gelatinase A activation" 50 : 183-186, 2001

11 Ruhul Amin, A.R., "Secretion of matrix metalloprotein-ase-9 by the proinflammatory cytokine, IL-1beta: a role for the dual signalling pathways" 8 : 515-523, 2003

12 Ho, J.N., "Protective effects of aucubin isolated from Eucommia ulmoides against UVB-induced oxidative stress in human skin fibroblasts" 28 : 1244-1248, 2005

13 Kim, J., "Protective effects of (-)-epigallo-catechin-3-gallate on UVA- and UVB-induced skin damage" 14 : 11-19, 2001

14 Sun, H.B., "Promoter competition assay for analyzing gene regulation in joint tissue engineering" 7 : a169-174, 2002

15 Tanaka, K., "Prevention of the ultraviolet B-mediated skin photoaging by a nuclear factor kappaB inhibitor, parthenolide" 315 : 624-630, 2005

16 Scharffetter-Kochanek, K, "Photoaging of the skin from phenotype to mechanisms" 35 : 307-316, 2000

17 Chung, J.H, "Photoaging in Asians" 19 : 109-121, 2003

18 Jung, C.H., "Phenolic-rich fraction from Rhus verniciflua Stokes (RVS) suppress inflammatory response via NF-kappaB and JNK pathway in lipopolysaccharide-induced RAW 264.7 macrophages" 110 : 490-497, 2007

19 Fisher, G.J., "Pathophysiology of premature skin aging induced by ultraviolet light" 337 : 1419-1428, 1997

20 Wenk, J., "Overexpression of phospholipid-hydroperoxide glutathione peroxid-ase in human dermal fibroblasts abrogates UVA irradiation-induced expression of interstitial collagenase/matrix metalloproteinase-1 by sup-pression of phosphatidylcholine hydroperoxide-mediated NFkappaB activation and interleukin-6 release" 279 : 45634-45642, 2004

21 Fisher, G.J., "Molecular basis of sun-induced premature skin ageing and retinoid antagonism" 379 : 335-339, 1996

22 Chung, J.H., "Modulation of skin collagen metabolism in aged and photoaged human skin in vivo" 117 : 1218-1224, 2001

23 Tanaka, K., "Magnolia ovovata extract and its active component magnolol prevent skin photoaging via inhibition of nuclear factor kappaB" 565 : 212-219, 2007

24 Weng, C.J., "Lucidenic acid inhibits PMA-induced invasion of human hepatoma cells through inactivating MAPK/ERK signal transduction pathway and reducing binding activities of NF-kappaB and AP-1" 29 : 147-156, 2008

25 Bell, S., "Involvement of NF-kappaB signalling in skin physiology and disease" 15 : 1-7, 2003

26 Varani, J., "Inhibition of type I procollagen production in photodamage: correlation between presence of high molecular weight collagen fragments and reduced procollagen synthesis" 119 : 122-129, 2002

27 Lee, J.C., "Identification of Rhus verniciflua Stokes compounds that exhibit free radical scavenging and anti-apopto-tic properties" 1570 : 181-191, 2002

28 Takao, J., "Expression of NF-kappaB in epidermis and the relationship between NF-kappaB activation and inhibition of keratinocyte growth" 148 : 680-688, 2003

29 Saliou, C., "Antioxidants modulate acute solar ultraviolet radiation-induced NF-kappa-B activation in a human keratinocyte cell line" 26 : 174-183, 1999

30 Park, K.Y., "Antimutagenic activity of flavonoids from the heartwood of Rhus verniciflua" Elsevier BV 90 (90): 73-79, 2004

31 Jeon, W.K., "Anti-platelet effects of bioactive compounds isolated from the bark of Rhus verniciflua Stokes" 106 : 62-69, 2006

32 Eberhardt, W., "Amplification of IL-1 beta-induced matrix metalloproteinase-9 expres-sion by superoxide in rat glomerular mesangial cells is mediated by increased activities of NF-kappa B and activating protein-1 and involves activation of the mitogen-activated protein kinase pathways" 165 : 5788-5797, 2000

33 Madrid, L.V., "Akt stimulates the transactivation potential of the RelA/p65 Subunit of NF-kappa B through utilization of the Ikappa B kinase and activation of the mitogen-activated protein kinase p38" 276 : 18934-18940, 2001

34 Chung, J.H., "Aging and photoaging" 49 : 690-697, 2003

35 Gilchrest, B.A., "Ageing and photoageing of the skin: observations at the cellular and molecular level" 127 (127): 25-30, 1992

36 Brenneisen, P., "Activation of p70 ribosomal protein S6 kinase is an essential step in the DNA damage-dependent signaling pathway responsible for the ultraviolet B-mediated increase in interstitial collagenase (MMP-1) and stromelysin-1 (MMP-3) protein levels in human dermal fibroblasts" 275 : 4336-4344, 2000

37 Gilchrest, B.A, "A review of skin ageing and its medical therapy" 135 : 867-875, 1996

38 Bradford, M.M, "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding" 72 : 248-254, 1976