Effect of PEG Addition on Pore Morphology and Biocompatibility of PLLA Scaffolds Prepared by Freeze Drying

다국어 초록 (Multilingual Abstract)

Purpose A 3wt% poly(L-lactic acid) (PLLA) with differentconcentrations of polyethylene glycol (PEG, 0~9wt%) wasblended and lyophilized to evaluate the morphology and thebiocompatibility of the PLLA/PEG scaffolds. The purpose ofthis study is to investigate the biocompatibility of the PLLA/PEG blends. Morphology, degradation rate, cytotoxicity, skinsensitization, acute systemic toxicity, and intradermal reactivityare examined.
Methods The morphology of the scaffolds was examined byusing scanning electron microscopy. The degradation of thescaffolds in phosphate buffer solution was measured for upto 9 weeks by measuring the weight loss. The extract testmethod was conducted on the scaffolds to evaluate the potentialof cytotoxicity on the base of the International Organizationfor Standardization (ISO 10993-5). Skin sensitization, acutesystemic toxicity, and intradermal reactivity were conductedaccording to ISO 10993-10(2010), ISO 10993-11(2006), ISO10993-10(2010), respectively.
Results The lamellar morphology of PLLA scaffold waschanged to the ladder-like structure with adding PEG. Thepore size of the PLLA/PEG blends decreased from 24±6 μmto 13±2 μm with increasing the PEG content from 0wt% to9wt%. As a result of the measurement, degradation rate rosewith increasing the PEG content in PLLA and biodegradablePLLA/PEG blend scaffolds exhibited excellent biocompatibilitydue to the absence of cytotoxicity, skin sensitizing potency,acute systemic toxicity, and intradermal reactivity.
Conclusions This outcome implied that the biodegradablePLLA/PEG scaffolds were clinically safe and effective.

참고문헌 (Reference)

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