An Alternative Approach to the Fermentation of Sweet Sorghum Juice into Biopolymer of Poly-β-hydroxyalkanoates (PHAs) by Newly Isolated, Bacillus aryabhattai PKV01

다국어 초록 (Multilingual Abstract)

This work revealed for the first time the possible use of a newly isolated Bacillus aryabhattai PKV01 for poly-β-hydroxyalkanoates (PHAs) production from fermentative sweet sorghum juice. Its growth and PHA production were investigated under different pH and nitrogen sources. Medium composition was optimized using statistical tools. The highest biomass and PHA content were reached at pH 6.5 with the use of urea. Plackett-Burman design was then applied to test the relative importance of medium components and process variables on cell growth and PHA production. Cell growth and PHAs production were affected by total sugar and urea and were subjected to optimize the sorghum juice medium using response surface methodology (RSM) via central composite design (CCD). The predicted optimal culture composition was achieved. Maximum dry cell weight and PHAs were obtained using a flask and almost double the amount was achieved using a bioreactor. After PHA recovery,the structure and thermal properties were characterised and revealed to be similar to the standard of poly-β-hydroxybutyrate (PHB).

참고문헌 (Reference)

1 Suwannasing, W., "Yields of Polyhydroxyalkanoates (PHAs) during batch fermentation of sugar cane juice by Alcaligenes latus and Alcaligenes eutrophus" 5 : 960-966, 2011

2 Liang, Y., "Use of sweet sorghum juice for lipid production by Schizochytrium limacinum SR21" 101 : 3623-3627, 2010

3 Hetényi, K., "Use of sweet sorghum juice for lactic acid fermentation: Preliminary steps in a process optimization" 85 : 872-877, 2010

4 Tanamool, V., "The Direct screening of potential polyhydroxylalkanoates (PHAs) bacterial from soil environment using sweet sorghum as a sole carbon source" 2011

5 Sipos, B., "Sweet sorghum as feedstock for ethanol production: Enzymatic hydrolysis of steam-pretreated bagasse" 153 : 151-162, 2009

6 Massoud, M. I., "Suitability of Sorghum bicolor L. stalks and grains for bioproduction of ethanol" 56 : 83-87, 2011

7 Khanna, S., "Statistical media optimization studies for growth and PHB production by Ralstoniaeutropha" 40 : 2173-2182, 2005

8 Mokhtari-Hosseini, Z. B., "Statistical media optimization for growth and PHB production from methanol by a methylotrophic bacterium" 100 : 2436-2443, 2009

9 Halami, P. M., "Production of polyhydroxyalkanoate from starch by the native isolate Bacillus cereus CFR06" 24 : 805-812, 2007

10 Yezza, A., "Production of poly-β-hydroxybutyrate (PHB) by Alcaligenes latus from maple sap" 77 : 269-274, 2007

11 Wu, Q., "Production of poly-3-hydroxybutyrate by Bacillus sp. JMa5 cultivated in molasses media" 80 : 111-118, 2001

12 Almodares, A., "Production of bioethanol from sweet sorghum: A review" 4 : 772-780, 2009

13 Gouda, M. K., "Production of PHB by a Bacillus megaterium strain using sugarcane molasses and corn steep liquour as sole carbon and nitrogen sources" 156 : 201-207, 2001

14 Sindhu, R., "Production and characterization of poly-3-hydroxybutyrate from crude glycerol by Bacillus sphaericus NII 0838 and improving its thermal properties by blending with other polymers" 54 : 783-794, 2011

15 Thirumala, M., "Production and characterization of PHB from two novel strains of Bacillus spp. isolated from soil and activated sludge" 37 : 271-278, 2010

16 Ramadas, N. V., "Polyhydroxybutyrate production using agro-industrial residue as substrate by Bacillus sphaericus NCIM 5149" 52 : 17-23, 2009

17 Philip, S., "Polyhydroxyalkanoates: Biodegradable polymers with a range of applications" 82 : 233-247, 2007

18 Valappil, S. P., "Polyhydroxyalkanoate (PHA) biosynthesis from structurally unrelated carbon sources by a newly characterized Bacillus spp" 127 : 475-487, 2007

19 Santimano, M. C., "PHA production using low-cost agro-industrial wastes by Bacillus sp. strain COL1/A6" 4 : 89-96, 2009

20 Faccin, D. J. L., "Optimization of C:N ratio and minimal initial carbon source for poly(3-hydroxybutyrate) production by Bacillus megaterium" 84 : 1756-1761, 2009

21 RamKumar Pandian, S., "Optimization and fed-batch production of PHB utilizing dairy waste and sea water as nutrient sources by Bacillus megaterium SRKP-3" 101 : 705-711, 2010

22 Anderson, A. J., "Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates" 54 : 450-472, 1990

23 Doi, Y., "Nuclear magnetic resonance studies on poly(β-hydroxybutyrate) and a copolyester of β-hydroxybutyrate and β-hydroxyvalerate isolated from Alcaligenes eutrophus H16" 19 : 2860-2864, 1986

24 Ishizaki, A., "Microbial production of poly-D-3-hydroxybutyrate from CO2" 57 : 6-12, 2001

25 Kominek, L. A., "Metabolism of polybeta- hydroxybutyrate and acetoin in Bacillus cereus" 90 : 1251-1259, 1965

26 Kulpreecha, S., "Inexpensive fed-batch cultivation for high poly(3-hydroxybutyrate) production by a new isolate of Bacillus megaterium" 107 : 240-245, 2009

27 Kim, P., "Improvement in cell yield of Methylobacterium sp. by reducing the inhibition of medium components for poly-β-hydroxybutyrate production" 19 : 357-361, 2003

28 Jiang, Y., "High poly(β-hydroxybutyrate) production by Pseudomonas fluorescens A2a5 from inexpensive substrates" 42 : 167-172, 2008

29 Grothe, E., "Fermentation optimization for the production of poly [beta]-hydroxybutyric acid) microbial thermoplastic" 25 : 132-141, 1999

30 Choi, J., "Factors affecting the economics of polyhydroxyalkaonoate production by bacterial fermentation" 51 : 13-21, 1999

31 Laopaiboon, L., "Ethanol production from sweet sorghum juice using very high gravity technology: Effects of carbon and nitrogen supplementations" 100 : 4176-4182, 2009

32 Nakata, H. M., "Effect of pH on Intermediates produced during growth and sporulation of Bacillus cereus" 86 : 577-581, 1963

33 Yüksekda , Z. N., "Effect of carbon and nitrogen sources and incubation times on poly-beta-hydroxybutyrate (PHB) synthesis by Bacillus subtilis 25 and Bacillus megaterium 12" 3 : 63-66, 2004

34 Yilmaz, M., "Determination of poly-β-hydroxybutyrate (PHB) production by some Bacillus spp" 21 : 565-566, 2005

35 Oliveira, F. C., "Characterization of poly(3-hydroxybutyrate) produced by Cupriavidus necator in solid-state fermentation" 98 : 633-638, 2007

36 Tanamool, V., "Biosynthesis of poly hydroxylalkanoate (PHA) by Hydrogenophaga sp. isolated from soil environments during batch fermentation" 5 : 1003-1012, 2011

37 Kaewkannetra, P., "Biorefinery of squeeze sweet sorghum juice into value added product of biopolymer" 136 : 412-, 2008

38 Antonopoulou, G., "Biofuels generation from sweet sorghum: Fermentative hydrogen production and anaerobic digestion of the remaining biomass" 99 : 110-119, 2008

39 Singh, M., "Bacillus subtilis as potential producerfor polyhydroxyalkanoates" 8 : 1-11, 2009

40 Law, J. H., "Assay of poly-beta-hydroxybutyric acid" 82 : 33-36, 1961

41 Mamma, D., "An alternative approach to the bioconversion of sweet sorghum carbohydrates to ethanol" 8 : 99-103, 1995

42 Palleroni, N. J., "Alcaligenes latus, a new species of hydrogen-utilizing bacteria" 28 : 416-424, 1978

43 Spiekermann, P., "A sensitive, viable-colony staining method using Nile red for direct screening of bacteria that accumulate polyhydroxyalkanoic acids and other lipid storage compounds" 171 : 73-80, 1999