Fluorescence has long been recognized as an important tool for probing biological structure and function. Development of optically active structures that can enhance fluorescence intensity through resonant near fields have gained much attention as a m...
Fluorescence has long been recognized as an important tool for probing biological structure and function. Development of optically active structures that can enhance fluorescence intensity through resonant near fields have gained much attention as a means for detecting fluorescent-tagged analystes at low concentrations for applications in DNA expression analysis and protein diagnostic assays. We fabricated Ag nanoparticles on a glass substrate by the oblique angle deposition method, which can fabricate highly porous film with a structure composed of isolated vertical nanorods with low cost. The fabricated device can enhance the fluorescence intensity due to the enhanced electromagnetic field around the Ag nanoparticles by the surface plasmon resonance effect. The fluorescence enhancement effects of the Cy-5 conjugated Streptavidin on the fabricated substrate were examined.