Extraction of phenolic compounds from Burdock root waste using ultrasonication as a green technology

다국어 초록 (Multilingual Abstract)

Burdock (Arctium lappa L.) roots are using as traditional medicine due to high content of bioactive compounds and antioxidant properties. In this study, ultrasonic extraction parameters such as solid to water ratio (20-40 g/ml), sonication time (60-100 min), and sonication temperature (40-60°C) were optimized using response surface methodology (RSM). After multiple linear regression analysis, a second-order polynomial model was developed for each response (reducing sugar and total phenolic content). The total phenolic compound was extracted as 1.0 to 3.75 mg GAE/g. It was observed that, solid to water ratio and sonication temperature have significant effects (p<0.05) on the extraction of phenolic compounds. Optimum extraction condition was found to be solid/water ratio: 39.98 g/ml, sonication time: 72.86 min, and sonication temperature: 59.99 °C. Additionally, LC-MS analysis confirmed the presence of various phenolic compounds such as tannins, caffeic acid derivatives and chlorogenic acids.