Transcription of Human Interleukin-2 Gene in Nicotiana tabacum Driven by caMV35S Promoter

다국어 초록 (Multilingual Abstract)

Human interleukin-2 (IL-2) gene was inserted next of the 35S-transcript promoter of cauliflower mosaic virus in a binary vector and introduced into tobacco genome by Agrobacterium-mediated transformation. Genomic DNA blot analysis of the in vitro regenerated transgenic tobacco confirmed that the introduced human IL-2 gene was successfully integrated into tobacco genome. RNA blot analyses were carried out for the IL-2 transcript to examine expression of the introduced IL-2 gene. Specific signals only from the transgenic tobacco plants indicated that the introduced human IL-2 gene was properly transcribed. The transgenic tobacco plants were self-pollinated and the seeds were harvested. Differences in the level of the transcript were more apparent during the germination of the seeds and between the T_2 generation tobacco plants.