Filamentous fungi Aspergillus species에서 Phytase 고발현 균주의 선별 = Screening of Phytase High-Expression Strains in Filamentous Fungi Aspergillus

다국어 초록 (Multilingual Abstract)

Phytase production of Aspergillus species were influenced by nitrogen source. Ammonium ions, especially ammonium phosphate (NH4H2PO4), were markedly superior to nitrate for the Phytase production. For the isolation of phytase gene, the culture of Aspergillus species (A. oryzae) were maintained on PSM media containing ammonium phosphate. After 4 days, mycellia were harvested by centrifugation and isolated total RNA. cDNAs were synthesized from total RNA by RT-PCR. The Phytase gene was amplified with the primers designed from A. ficuum sequence. 1.2 and 2.0 kb of the amplified DNA fragments by secondary PCR and subcloned in pT7Blue-T vector.
Aspergillus species (A. oryzae and A. ficuum) were treated with 254㎚ of UV light for the screening of high phytase producing mutant strains, comparative to wild type on PSM media, containing ammonium phosphate. Two UV mutant strains in A. oryzae and three, in A. ficuum were isolated. These mutants are 1.5 to 2.0 times better phytase-production than Wild type.

목차 (Table of Contents)

• Ⅰ서론
• Ⅱ.실험 재료 및 방법
• 1.균주 및 배지
• 2.Phytase 유전자의 분리 및 동정
• 3.UV Mutation에 의한 고발현 돌연변이 주의 선별
• Ⅲ.결과 및 논의
• 1.Phytase 유전자의 분리 및 동정
• 2.UV Mutation에 의한 고발현 돌연변이 주의 선별
• Ⅳ.결론