저칼슘 식이로 유발한 성장 저하 흰쥐에 대한 칼슘, 비타민 D 및 난황 펩타이드의 투여가 장골 길이 성장에 미치는 영향 = Effects of Calcium, Vitamin D and Egg Yolk Peptide Treatment on the Retardation of Longitudinal Bone Growth Induced by Low-Calcium Diets

다국어 초록 (Multilingual Abstract)

Objectives : Egg yolk is composed of various important chemical substances for human health. A calcium shortage causes the growth retardation on the body growth. In this study, we examined the therapeutic effects of calcium, vitamin D and egg yolk peptide (EYP) treatment on the retardation of the longitudinal bone growth induced by low-calcium diet in adolescent rats. Methods : Low calcium diets were administrated for 15 days. During the last five days, calcium and/or vitamin D and/or EYP were administrated. The body weights, longitudinal bone growth rates, the heights of growth plates, and bone morphogenetic protein (BMP)-2 and insulin-like growth factor (IGF)-1 expressions were measured using histochemical analysis. Results : Low calcium diets caused the significant reduction in body weight gains and the longitudinal bone growth. The heights of growth plates and the expressions of BMP-2 and IGF-1 showed the impairment of body growth as well. Calcium and/or vitamin D administration could not significantly increase the longitudinal bone growth. However, calcium, vitamin D, and EYP administration significantly increased the bone growth, the growth plate height, and BMP-2 and IGF-1 expressions. Conclusions : These results suggest that EYP enhances the longitudinal bone growth in the calcium and/or vitamin D deficiency and it could be a promising agent for the treatment of children suffering from malnutrition.

참고문헌 (Reference)

1 임강현, "성장관련 한약 혼합제제의 흰쥐 장골 길이성장에 대한 효과" 대한본초학회 18 (18): 20-186, 2003

2 최희정, "비타민 D 작용에 대한 새로운 조명" 대한가정의학회 32 (32): 89-96, 2011

3 SATCM, "Zhong Hua Ben Cao vol 9. 1st ed" Shanghai Science and Technology Press 477-478, 1999

4 Li ZZ, "Yi Zong Bi Du. 1st ed" Tree and earth press 22-24, 2000

5 Chris Clarke, "The science of ice cream" Royal Society of Chemistry 49-, 2004

6 Scriver CR, "The Metabolic and Molecular Bases of Inherited Disease. Vol. II. 7th ed" McGraw-Hill 3023-3044, 1995

7 Korean P, "Structure, composition, and formation of egg" 174 : 54-57, 2004

8 Wang EA, "Recombinant human bone morphogenetic protein induces bone formation" 87 : 2220-2224, 1990

9 Kim HK, "Protective effect of egg yolk peptide on bone metabolism" 18 (18): 307-313, 2011

10 Gerard JT, "Principles of anatomy &physiolosy. 10th ed" Wiley 174-, 2003

11 Frank H Netter, "Musculoskeletal system: anatomy, physiology, and metabolic disorders. 1st ed" Ciba-Geigy Corporation 130-, 1987

12 Holick MF, "Isolation and identification of 1,25-dihydroxycholecalciferol. A metabolite of vitamin D active in intestine" 10 (10): 2799-2804, 1971

13 Fiorito LM, "Girls' calcium intake is associated with bone mineral content during middle childhood" 136 : 1281-1286, 2006

14 Hwang KS, "Functional properties and commercial application of lecithin" 17 : 303-319, 1995

15 Mackie EJ, "Endochondral ossification: how cartilage is converted into bone in the developing skeleton" 40 (40): 46-62, 2008

16 Forsyhe RH, "Eggs and Egg products as functional ingredients" 8 : 4-46, 1970

17 Leem KH, "Effects of egg yolk proteins on the longitudinal bone growth of adolescent male rats" JAPAN SOC BIOSCI BIOTECHN AGROCHEM 68 (68): 2388-2390, 2004

18 Chen H, "Effect of low or high dietary calcium on the morphology of the rat femur" 17 (17): 1129-1135, 2002

19 Heo J, "Donguibogam. 1st ed" Bubin publishers co 1860-, 2005

20 Kim DG, "Dongui pediatry. 1st ed" Jungdam press 353-, 2002

21 Catharine R, "Dietary Reference Intakes for Calcium and Vitamin D. 1st ed" National Academy Press 35-74, 2011

22 Brighton CT, "Cytoplasmic structures of epiphyseal plate chondrocytes;quantitative evaluation using electron micrographs of rat costochondral junctions with specific reference to the fate of hypertrophic cells" 55 (55): 771-784, 1973

23 Bonjour JP, "Consumption of vitamin D-and calcium-fortified soft white cheese lowers the biochemical marker of bone resorption TRAP 5b in postmenopausal women at moderate risk of osteoporosis fracture" 142 : 698-703, 2012

24 Cancedda R, "Chondrocyte differentiation" 159 : 265-358, 1995

25 Matkovic V, "Calcium supplementation and bone mineral density in females from childhood to young adulthood: a randomized controlled trial" 81 : 175-188, 2005

26 Forriol F, "Bone development: interaction of molecular components and biophysical forces" 432 : 14-33, 2005

27 Hallahan AR, "BMP-2 mediates retinoid-induced apoptosis in medulloblastoma cells through a paracrine effect" 9 : 1033-1038, 2003

28 Hollis BW, "Assessment of vitamin D nutritional and hormonal status: what to measure and how to do it" 58 (58): 4-5, 1996

29 Abdou AM, "Antiosteoporotic effect of orally administered yolk-derived peptides on bone mass in women" 2 (2): 193-199, 2014