Toxin–antitoxin (TA) systems are ubiquitous among most of prokaryotes and govern the cell death or growth arrest in response to environmental cues. TA systems are associated with adaptation of pathogens to unfavorable environments, indicating t...
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https://www.riss.kr/link?id=A105276948
2018
-
472
KCI등재후보
학술저널
23-27(5쪽)
0
상세조회0
다운로드다국어 초록 (Multilingual Abstract)
Toxin–antitoxin (TA) systems are ubiquitous among most of prokaryotes and govern the cell death or growth arrest in response to environmental cues. TA systems are associated with adaptation of pathogens to unfavorable environments, indicating t...
Toxin–antitoxin (TA) systems are ubiquitous among most of prokaryotes and govern the cell death or growth arrest in
response to environmental cues. TA systems are associated with adaptation of pathogens to unfavorable environments, indicating their potential as a target for antibiotics. Here, we purified and crystallized the TA complex from the extremophile Deinococcus radiodurans. The TA complex (DrMazEF) was co-expressed and pulled using the N-terminal glutathione S-transferase-tagged DrMazF. The complex was crystallized in 100 mM citric acid pH 3.5 containing 25% PEG3350. The crystal diffracted X-ray to a 2.6 Å resolution and belonged to the space group P212121, with the unit cell parameters a =46.01, b = 74.04, and c = 138.26 Å. The asymmetric unit of the crystal had six molecules in two heterotrimeric complexes with a calculated Mathew’s coefficient of 1.84 Å3 Da-1 and a solvent content of 33.18%.
목차 (Table of Contents)
Structural aspects of EF-hand containing calcium buffers, sensors, and S100 family
Application of helix fusion method in structural biology
Time-resolved serial femtosecond X-ray crystallography