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    Correlation Analysis between Blood and Hair Cortisol Concentrations in Hanwoo Due to Transportation

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    https://www.riss.kr/link?id=A108848671

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    다국어 초록 (Multilingual Abstract) kakao i 다국어 번역

    Animal welfare has been gradually gathering more attention from consumers over time, making it increasingly important to assess the level of stress experienced by livestock. Traditionally, stress has been measured by collecting blood to assess cortisol levels, an action that can be considered distressing for the animal. Therefore, we aimed to explore the feasibility of using hair as an alternative medium to blood for stress assessment. We utilized B/F (blood collected at the farm), B/A (blood collected after transport to the auction), and H/A (hair collected at the auction after blood sampling) from calves at the age of 7-9 months transported from the farm to the auction. Hair underwent a washing and extraction process to utilize hair extracts, while blood was centrifuged to analyze using ELISA. The cortisol concentration in the blood was significantly higher in B/A compared to B/F (p<0.05), confirming that the calves experienced stress during transportation. Additionally, H/A was significantly lower than both B/A and B/F (p<0.0001). These results emphasized that cortisol in hair is not suitable for investigating short-term stress in livestock, as it is with blood. While measuring stress indices using hair may not be appropriate for replacing blood, it is considered a highly suitable practice for animal welfare, and further research in this area should be continued
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    Animal welfare has been gradually gathering more attention from consumers over time, making it increasingly important to assess the level of stress experienced by livestock. Traditionally, stress has been measured by collecting blood to assess cortiso...

    Animal welfare has been gradually gathering more attention from consumers over time, making it increasingly important to assess the level of stress experienced by livestock. Traditionally, stress has been measured by collecting blood to assess cortisol levels, an action that can be considered distressing for the animal. Therefore, we aimed to explore the feasibility of using hair as an alternative medium to blood for stress assessment. We utilized B/F (blood collected at the farm), B/A (blood collected after transport to the auction), and H/A (hair collected at the auction after blood sampling) from calves at the age of 7-9 months transported from the farm to the auction. Hair underwent a washing and extraction process to utilize hair extracts, while blood was centrifuged to analyze using ELISA. The cortisol concentration in the blood was significantly higher in B/A compared to B/F (p<0.05), confirming that the calves experienced stress during transportation. Additionally, H/A was significantly lower than both B/A and B/F (p<0.0001). These results emphasized that cortisol in hair is not suitable for investigating short-term stress in livestock, as it is with blood. While measuring stress indices using hair may not be appropriate for replacing blood, it is considered a highly suitable practice for animal welfare, and further research in this area should be continued

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    참고문헌 (Reference)

    1 Damtew, A., "The effect of long distance transportation stress on cattle : a review" 2 (2): 2018

    2 Novak, M. A., "Stress, the HPA axis, and nonhuman primate well-being : A review" 143 (143): 135-149, 2013

    3 Wennig, R., "Potential problems with the interpretation of hair analysis results" 107 (107): 5-12, 2000

    4 Thau, L., "Physiology, Cortisol" Stat Pearls Publishing 2022

    5 Palme, R., "Monitoring stress hormone metabolites as a useful, non-invasive tool for welfare assessment in farm animals" 21 (21): 331-337, 2012

    6 Meyer, J. S., "Minireview : Hair Cortisol : A Novel Biomarker of Hypothalamic-Pituitary-Adrenocortical Activity" 153 (153): 4120-4127, 2012

    7 El-Farhan, N., "Measuring cortisol in serum, urine and saliva are our assays good enough?" 54 (54): 308-322, 2017

    8 Bennett, A., "Measuring cortisol in hair and saliva from dogs : coat color and pigment differences" 39 (39): 171-180, 2010

    9 Salaberger, T., "Influence of external factors on hair cortisol concentrations" 233 : 73-78, 2016

    10 Ito, N., "Human hair follicles display a functional equivalent of the hypothalamicpituitary-adrenal(HPA)axis and synthesize cortisol" 19 (19): 1332-1334, 2005

    1 Damtew, A., "The effect of long distance transportation stress on cattle : a review" 2 (2): 2018

    2 Novak, M. A., "Stress, the HPA axis, and nonhuman primate well-being : A review" 143 (143): 135-149, 2013

    3 Wennig, R., "Potential problems with the interpretation of hair analysis results" 107 (107): 5-12, 2000

    4 Thau, L., "Physiology, Cortisol" Stat Pearls Publishing 2022

    5 Palme, R., "Monitoring stress hormone metabolites as a useful, non-invasive tool for welfare assessment in farm animals" 21 (21): 331-337, 2012

    6 Meyer, J. S., "Minireview : Hair Cortisol : A Novel Biomarker of Hypothalamic-Pituitary-Adrenocortical Activity" 153 (153): 4120-4127, 2012

    7 El-Farhan, N., "Measuring cortisol in serum, urine and saliva are our assays good enough?" 54 (54): 308-322, 2017

    8 Bennett, A., "Measuring cortisol in hair and saliva from dogs : coat color and pigment differences" 39 (39): 171-180, 2010

    9 Salaberger, T., "Influence of external factors on hair cortisol concentrations" 233 : 73-78, 2016

    10 Ito, N., "Human hair follicles display a functional equivalent of the hypothalamicpituitary-adrenal(HPA)axis and synthesize cortisol" 19 (19): 1332-1334, 2005

    11 Comin, A., "Hair cortisol levels in dairy cows from winter housing to summer highland grazing" 138 (138): 69-73, 2011

    12 Tallo-Parra, O., "Hair cortisol detection in dairy cattle by using EIA : protocol validation and correlation with faecal cortisol metabolites" 9 (9): 1059-1064, 2015

    13 Russell, E., "Hair cortisol as a biological marker of chronic stress : Current status, future directions and unanswered questions" 37 (37): 589-601, 2012

    14 Wester, V. L., "Hair cortisol and cortisone are decreased by natural sunlight" 72 : 94-96, 2016

    15 Kirschbaum, C., "Hair as a retrospective calendar of cortisol production —Increased cortisol incorporation into hair in the third trimester of pregnancy" 34 (34): 32-37, 2009

    16 Meyer, J., "Extraction and Analysis of Cortisol from Human and Monkey Hair" (83) : e50882-, 2014

    17 Hamel, A. F., "Effects of shampoo and water washing on hair cortisol concentrations" 412 (412): 382-385, 2011

    18 Del Rosario, G. D. L. V., "Effects of adrenocorticotropic hormone challenge and age on hair cortisol concentrations in dairy cattle" 75 (75): 216-221, 2011

    19 Schlake, T., "Determination of hair structure and shape" 18 (18): 267-273, 2007

    20 Braun, U., "Cortisol concentration of regrown hair and hair from a previously unshorn area in dairy cows" 114 : 412-415, 2017

    21 Ghassemi Nejad, J., "Coat and hair color : hair cortisol and serotonin levels in lactating Holstein cows under heat stress conditions" 88 (88): 190-194, 2017

    22 Fazio, E., "Circulating β-endorphin, adrenocorticotrophic hormone and cortisol levels of stallions before and after short road transport : stress effect of different distances" 50 (50): 6-, 2008

    23 Angelousi, A., "ACTH Action on the Adrenals" MDText.com, Inc 2000

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