In vitro Regeneration of potato (Solanum tuberosum cv. Jopung) transformants

다국어 초록 (Multilingual Abstract)

Efficient plant regeneration has been achieved in the potato plant (Solanum tuberosum cv. Jopung). We found that a modified medium was given shoot regeneration within 10 weeks after explants infected with A. tumefaciens were cultured. Callus appeared at the cut edges of tuber discs and of stem segments, mainly at the basal parts calli were observed. Some explants started to form shoots and roots after two to three weeks in stage Ⅲ medium. They were then transferred to MS medium containing 200 ㎎/L kanamycin. The transformed shoots formed roots at the cut edge of the plantlets. In contrast. untransformed shoots did not form roots and became yellowish after a month in the same medium. In conclusion, we improved in vitro regeneration and transformation of potato using agrobacteria harboring β-glucuronidase (GUS) and modified media.