남부지방 수집종(蒐集種) 차나무(Camellia sinensis L.) 유연관계 분석 = Analysis of Genetic Relationship of Collected Teas (Camellia sinensis L.) from Southern Part of Korea

다국어 초록 (Multilingual Abstract)

The investigation on the morphological and genetic relationship was carried out with leaves of teas(Camellia sinenesis L.) which were collected from 16 southern areas of Korea including one Japanese location: Hadong, Sachun, Gosueng, Namhae, Milyang, Gimhae, Busan, Jinju, Yangsan, Gurye, Suncheon, Boseong, Gangjin, Haenam, Jeju and Shizhuoka(Japan).
In the analysis of genetic relationship, RAPD analysis was done with 30 primers purchased at Bioneer company. There were 80 expanded bands totally observed and 58 bands were found to have polymorphism. The size of expanded DNA was between 200bp and 2,000bp. For the analysis of genetic relationship, NTSYS computer program was used and the similarity value among domestic green teas was 0~0.09. With the similarity value matrix, 12 groups were formed as a result of 16 kinds of dendrogram. The similarity value was so high between Sachun and Gosueng, and between Gurye and Suncheon as 0.90. In the cases of Sachun and Gosueng, there was no difference in leaf length, leaf shape, and leaf width (data not shown) indicating the presence of coincidence between RAPD analysis and morphological analysis. However, in the cases of Gurye and Suncheon, morphological characteristics was not coincident with DNA analysis where both collections belong to same group. Using the polymorphic bands which has a distinct presence or absence in RAPD analysis, the SCAR marker was constructed. Among the 5 SCAR markers, only one from RAPD marker #4 showed a single amplification of 868bp indicating low efficiency of transformation rate from RAPD marker to SCAR marker. But removal of all multiple bands in SCAR amplification clearly revealed that SCAR marker is repetitive and reliable in the amplification of polymorphic DNA region.

목차 (Table of Contents)

• ABSTRACT
• 서 론
• 재료 및 방법
• 결과 및 고찰
• 참고문헌