Two new dammarane-type triterpene saponins from Korean red ginseng and their anti-inflammatory effects

다국어 초록 (Multilingual Abstract)

<P><B>Abstract</B></P> <P> <I>Panax ginseng</I> has been the subject of extensive research on potential medicinal materials. The goal of this study was search the chemical constituents and biological activities of processed <I>Panax ginseng</I>, Korean red ginseng. Our efforts led to the isolation eleven compounds (<B>1</B>–<B>11</B>) including two new compounds <B>1</B> and <B>2</B> from Korean red ginseng using various chromatographic techniques. Chemical structures of isolated compounds were demonstrated by spectroscopic methods (1D-, 2D-NMR, and HR-ESI-MS). The anti-inflammatory effects of the compounds were investigated by inhibiting IL-6 and TNF-α secretion in LPS-activated RAW264.7 cells. Additionally, the effects of the compounds on the expression of COX-2 and iNOS were examined by Western blotting. Compound <B>1</B> significantly reduced the level of proinflammatory cytokines IL-6 and TNF-α secretion in LPS-activated RAW264.7 cells and the expression of COX-2 and iNOS inflammatory enzymes in the cells. These results suggested that compound <B>1</B>, a new ginsenoside might useful in treatment of inflammation.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>