Ultra-high sensitive detection of Exosomal miRNAs based on Surface-Enhanced Raman Scattering = Ultra-high sensitive detection of Exosomal miRNAs based on Surface-Enhanced Raman Scattering

다국어 초록 (Multilingual Abstract)

Recent studies have shown that exosomal miRNAs have an important role in carcinogenesis by targeting cellular growth pathways that enhance intercellular communication and promote metastasis in cancers. Furthermore, miRNAs in exosome are circulating stably in body fluids. As such, exosomal miRNAs have become an ideal and non-invasive biomarker for the early diagnosis and prognosis. Herein, we developed an ultra-high selectivity and sensitivity nanoplasmonic biosensor based on surface-enhanced Raman scattering (SERS) using head-flocked gold nanopillar substrate for the attomolar detection of cancer-derived exosomal miRNA without any amplification step. In addition, this system could discriminate between miRNAs with high sequence similarities, even a single base mismatch. We anticipate that proposed biosensor offers great promise for the precise diagnosis and prognosis of cancer in clinical fields.