公害原으로서 水銀이 組織代謝에 미치는 影響 = The effect of mercury as a pollutant on tissue metabolism

국문 초록 (Abstract)

생후 2개월 내외 체중 150∼200g의 숫 흰쥐 50마리를 사용하여 적출한 신, 간, 고환, 대외, 횡격막, 심근 등 조직의 산소 소비향에 미치는 수은 및 승홍의 영향을 Umbreit의 manometer수법에 의하여 측정하였다.
신의 피질과 간은 수은 증기에 의하여 산소 소비량이 증가하는 경향이 있었으나 신의 수질, 고환, 대외피질, 횡격막, 심근 등은 도리어 유의의한 감소를 보였다.
승홍의 투여에 의하여서는 간과 신피질 다 같이 5x10^-7M에서 실험군의 산소 소비량이 대조군에 비하여 133.6%, 127%로 유의의하였으며, 횡격막은 5x10^-10M, 5x10^-9M에서 110%이었으나 유의의하지는 않았다. 대외피질은 산소 소비량이 억제되었다. 또한 승홍은 간의 Succinic dehydrogenase활성을 가소시키는 반면 Cytochrome oxidase활성은 5x10^-4M에서 유의의한 상승을 나타내었다.

다국어 초록 (Multilingual Abstract)

Mercury vapor and mercuric compounds have a toxic effect on living organs. They inhibit respiratory enzyme activities. This experiment was performed to study the tissue oxygen consumption affected by mercury vapor and mercuric chloride.
The 65 experimental animals were male rats weighing 150∼200g. The rats were sacrificed by decapitation, and their kidney, liver, heart, diaphragm, brain and testis were removed. They were divided into an experimental group whose tissue was ex-posed to mercury vapor and mercuric chloride, and a control group. The oxygen consumption of each organ was observed at 37℃, in vitro, by Warburg's manometric method.
The results obtained were summarized as follows:
(1) Under metallic mercury vapor, QO_2 of renal cortex and liver was higher in the experimental group than in the control group by 12.8% and 1.5% respectively. On the other hand, the QO_2 of renal medulla (9%), cerebral cortex (12%), dia-phragm (11%), heart (12%) and testis (10%) were significantly lower in the ex-perimental group than in the control group.
(2) At the concentration of 5 X 10^-7 Mol mercuric chloride, oxygen consumption of renal cortex (127.5%) and liver slice (133.6%) in the experimental group was significantly higher than in the control group.
(3) Oxygen consumption of diaphragm in the experimental group was slightly in-creased at the concentration of 5 X 10^-10 Mol and 5 X 10^-9 Mol mercuric chloride compared with the control group. But the oxygen consumption of cerebral cortex in the experimental group was significantly decreased at all the concentrations of mercuric chloride.
(4) Although the activities of Succinic dehydrogenase were inhibited by mercuric chloride, the Cytochrome oxidase was significantly increased at the concentration of 5 X 10^-4 Mol.