임신 전 부모의 운동이 새끼 쥐 해마의 신경가소성과 미토콘드리아 기능에 미치는 영향 = Effect of Parental Exercise before Pregnancy on Neuronal Plasticity and Mitochondrial Function in the Hippocampus of Offspring

국문 초록 (Abstract)

본 연구에서는 임신 전 부모의 운동이 태아의 인지기능, 해마의 미토콘드리아 기능, 그리고 신경영양인자에 어떠한 영향을 미치는지 알아보는 것이다. 부모가 될 암, 수 쥐를 비운동군과 운동군으로 구분하여 운동군은 8주간 트레드밀 운동을 실시하였다. 8주간 트레드밀 운동은 주 6회 실시하였으며, 운동처치로 인한 체력의 증가를 고려해, 속도(10 ~ 15 m/min)와 시간(30 ~ 40 min)을 점차적으로 증가시켰다. 트레드밀 운동을 시킨 뒤, 암수 한쌍 씩을 각각 i) 통제집단, ii) 어미 운동집단, iii) 아비 운동집단 그리고 iv) 부모 운동집단으로 분류하여 이틀간 교배를 실시하였다. 각 집단에서 태어난 새끼 쥐들은 10마리씩 표집하였으며, 생후 28일째 되는 날 인지기능을 확인하기 위하여 수중미로검사를 실시하였다. 본 연구결과, 부모 중 한쪽이라도 운동을 한 집단의 새끼들은 통제집단보다 인지기능, 해마의 미토콘드리아 기능(산소섭취량), 미토콘드리아 생합성, 그리고 신경영양인자가 증가하였다(p<0.05). 특히 어미, 또는 아비 하나만 운동한 경우보다 부모가 모두 운동한 경우가 더 높은 인지기능 및 미토콘드리아 기능을 나타내었다(p<0.05). 결론적으로, 임신 전 부모의 규칙적인 운동은 태아의 뇌기능 및 발달에 긍정적인 영향을 미칠 것으로 생각된다.

다국어 초록 (Multilingual Abstract)

In the present study, we investigated the effects of parental exercise before pregnancy on cognitive ability, mitochondrial function, and neurotrophic factor in hippocampus of offspring . The adult male and female rats were randomly divided into two groups respectively: control group and exercise group. The animals in the exercise groups were forced to run on a motorized treadmill for 8 weeks. The 8-week exercise training protocol consisted of running on a motorized treadmill six times per week with an incremental increase in treadmill velocity(10 ~ 15 m/min) and run duration(30 ~ 40 min) to accommodate increased fitness. After exercise treatment, the pairs of male and female rat were divided into four groups and were mated for 2 days: i) Control group, ii) Maternal exercise group, iii) Paternal exercise group and iv) Parental exercise group. Ten offsprings from each group were selected. On the 28th day after birth, Morris water maze task was performed to evaluated cognition. In the present study, maternal and/or paternal exercise increased cognitive ability, mitochondrial function(O₂ consumption), biogenesis and neurotrophic factor in the hippocampus of offspring(p<0.05). In addition, cognitive ability and mitochondrial function in the offspring from parental exercise group were higher than maternal or paternal exercise group(p<0.05). Based on these results, parental exercise before pregnancy may be effective at brain function and development in offsprings.

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