추출조건이 chitosan의 제조에 미치는 영향 = An Evaluation of Alkali and Acid Treatments for Chitosan Extraction from Aspergillus oryzae

다국어 초록 (Multilingual Abstract)

Chitosan have been usually prepared by deacetylation of chitin derived from Crustacea species such as lobster and crab with cone. NaOH. However, such treatment not only deacetylation N-acetyl glucosamine residues but also causes degradation of glycosidic bonds of chitins, decreasing their molecular weight. Some fungi contains as components of their cell walls. If chitosans prepared from these fungi have unique properties compared with those derived from Crustacea species, they would be valuable for industrial use.
Chitosan was isolated from Aspergillus oryzae. This can be the potential resource for the production of chitosan. The composition of A. oryzae has been investigated. The chemical analysis of A. oryzae was consists chiefly of carbohydrate(42.8%) and protein(42.5%), with smaller amounts of moisture(6.4%), ash(4.9%) and crude fat(3.4%). In the case of isolating of chitosan from A. oryzae, hot alkali extraction(40% NaOH, 120℃, Ihr.) was carried out to remove alkali soluble glucan, protein and lipid. This was followed by acetic acid extraction(0.5 N acetic acid, 95℃, 12hr.) to extract the chitosan. To isolated chitosan from A. oryzae and the affect of the yield of chitosan-glucan complex and glucosamine, the alkaline concentration, the temperature, and the extraction time were examined by ANOVA test. At the 5% significant level, the yield of chitosan-glucan complex was only affected by the extraction time while the glucosamine content was affected by the temperature. The yield of chitosan and chitosan-glucan complex were 5.89%(dry basis) and 20.73%(dry basis) of homogenized A. oryzae, respectively.

목차 (Table of Contents)

• 목차
• Abstract = 101
• Ⅰ. 서론 = 102
• Ⅱ. 실험재료와 방법 = 103
• 1. 사용균주 및 배양 = 103
• 2. A. oryzae mycelium의 성분 조건 = 103
• 3. A. oryzae로부터 chitosan의 제조 = 104
• 4. 추출조건의 chitosan-glucan복합체의 중량 및 glucosamine 함량에 미치는 효과 = 104
• Ⅲ. 실험결과 및 고찰 = 104
• 1. Chitosan원료의 성분분석 = 104
• 2. Aspergillus oryzae로부터 분리된 chitosan-glucan 복합체의 중량과 glucosamine의 함량 = 105
• 3. Chitosan 추출조건 = 106
• Ⅳ. 결론 = 107
• 참고문헌 = 109