잡초벼 PBR 혐기발아 내성 유전자 활용 벼 담수직파 초기 입모 개선 = Improvement of Seedling Establishment in Wet Direct Seeding of Rice using the Anaerobic Germination Tolerance Gene Derived from Weedy Photoblastic Rice

다국어 초록 (Multilingual Abstract)

Direct seeding is one of the rice seedling establishment methods that is increasingly being practiced by farmers to save labor and reduce costs. However, this method often causes poor germination under flooding conditions after sowing. In this study, we developed japonica elite lines with quantitative trait loci (QTL) associated with anaerobic germination (AG) tolerance to overcome poor germination and seedling establishment in wet direct seeding. The QTL introgression lines were developed from a cross between weedy photoblastic rice as the AG donor and the Nampyeong variety via phenotypic and genotypic selection.
Compared to Nampyeong, the survival rates of the selected lines were improved by approximately 50% and 240% under field and greenhouse conditions, respectively. To improve selection efficiency by marker assisted selection, the QTL markers associated with AG tolerance were converted to cleaved amplified polymorphic sequence markers designed based on next-generation sequence analysis. These lines retained similar agronomic traits and yield potential to the parent, Nampyeong. Among these lines, we selected the most promising line, which exhibited high survival rate and good agricultural traits under flooding conditions and named the line as Jeonju643. This line will contribute to breeding programs aiming to develop rice cultivars adapted to wet direct seeding. This study demonstrates the successful application of marker-assisted selection to targeted introgression of anaerobic genes into a premium quality japonica rice variety.

국문 초록 (Abstract)

본 연구에서는 국내 벼 직파재배 활성화 및 재배면적 확대를 위하여 담수발아 관련 QTLs 단편이 이입된 자포니카형 우량 계통을 육성하였다. 더불어 담수 중 혐기발아 내성과 관련 분자표지를 개발하였으며, 이를 우량계통 선발에직접 활용하였다. 인공교배를 통해 국내 자포니카 잡초벼인 PBR을 수여친으로 남평을 반복친으로 여교잡을 수행하였으며, 이 조합으로부터 담수 혐기 발아성이 개선된 자포니카 형 우량계통을 육성하였다. 그리고 혐기 발아 내성 관련 QTL 판별을 위한 CAPS 분자표지(NP01.014, NP03.077, NP11.091)를 개발하였다. 여교잡 집단을 육성하고, 작물학적 특성, 담수저항성 검정 등의 표현형 선발과 분자표지 선발을 통해 주요 농업형질, 담수 혐기 발아성이 양호한 5계통이 최종 선발되었으며, 이들 계통은 모두 QC1 혹은 QC2 조합으로 남평에 비해 온실에서 평균 2.3배, 포장에서 2.6 배 가량 유묘 생존율이 개선된 것으로 나타났다. 선발된 5 계통에 대한 생산력 검정 예비시험(PYT), 주요 작물학적특성 및 담수 혐기 발아성 등의 검정을 통해 28708을 담수직파 우량계통으로 최종 선발하고 ‘전주643호’로 계통명을 부여하였다.

참고문헌 (Reference)

1 김성은, "직파재배 벼와 화본과 잡초의 혐기 저온 내성 차이" 한국잡초학회 26 (26): 323-329, 2006

2 신운철, "벼 중생 고품질 직파 및 이앙 재배 겸용 ‘수안’" 한국육종학회 45 (45): 393-398, 2013

3 Seshu, D., "Seed vigor in rice" Manila 1988

4 Yamauchi, M., "Rice seed vigor and seedling establishment in anaerobic soil" 36 : 680-686, 1996

5 Magneschi, L., "Rice germination and seedling growth in the absence of oxygen" 103 (103): 181-196, 2009

6 Angaji, S. A., "QTLs associated with tolerance of flooding during germination in rice (Oryza sativa L.)" 172 (172): 159-168, 2010

7 Baltazar, M. D., "QTL mapping for tolerance of anaerobic germination from IR64 and the aus landrace Nanhi using SNP genotyping" 197 (197): 251-260, 2014

8 Jeong, J. M., "QTL mapping and effect confirmation for anaerobic germination tolerance derived from the japonica weedy rice landrace PBR" 139 (139): 83-92, 2020

9 Septiningsih, E. M., "QTL mapping and confirmation for tolerance of anaerobic conditions during germination derived from the rice landrace Ma-Zhan Red" 126 (126): 1357-1366, 2013

10 Singh, S., "Pyramiding three bacterial blight resistance genes (xa5, xa13and Xa21) using marker-assisted selection into indica rice cultivar PR106" 102 (102): 1011-1015, 2001

11 Pradhan, S. K., "Pyramiding of three bacterial blight resistance genes for broad-spectrum resistance in deepwater rice variety" 8 (8): 1186-, 2015

12 Dokku, P., "Pyramiding of four resistance genes of bacterial blight in Tapaswini, an elite rice cultivar, through marker-assisted selection" 192 (192): 87-96, 2013

13 Biswas, J. K., "Mechanism of seedling establishment of direct-seeded rice (Oryza sativa L.) under lowland conditions" 38 : 29-32, 1997

14 Biswas, J., "Mechanism of seedling establishment of direct-seeded rice (Oryza sativa L.) under lowland conditions" (38) : 29-32, 1997

15 Sundaram, R., "Marker assisted introgression of bacterial blight resistance in Samba Mahsuri, an elite indica rice variety" 160 : 411-422, 2008

16 RDA (Rural Development Administration), "Manual for standard evaluation method in agricultural experiment and research" RDA Press 2012

17 Awika, J. M, "Major cereal grains production and use around the world" 1089 : 1-13, 2011

18 Perez, L. M., "Introgression of Xa4, Xa7 and Xa21 for resistance to bacterial blight in thermosensitive genetic male sterile rice (Oryza sativa L.) for the development of two-line hybrids" 164 (164): 627-636, 2008

19 Tuong, T. P., "Increasing water productivity and weed suppression of wet seeded rice : Effect of water management and rice genotypes" 36 (36): 71-89, 2000

20 Kim, S. M., "Identification of QTLs for tolerance to hypoxia during germination in rice" 214 (214): 2018

21 Al-Ani, A., "Germination, Respiration, and Adenylate Energy Charge of Seeds at Various Oxygen Partial Pressures" 79 (79): 885-890, 1985

22 Pandey, S., "Direct seeding:research strategies and opportunities" International Rice Research Institute 3-14, 2002

23 Kumar, V., "Direct Seeding of Rice. Recent Developments and Future Research Needs" 111 : 297-413, 2011

24 Ismail, A. M., "Adaptation to flooding during emergence and seedling growth in rice and weeds, and implications for crop establishment" 2012 : pls019-pls019, 2012