Effect of three different irrigation solutions applied by passive ultrasonic irrigation

다국어 초록 (Multilingual Abstract)

Objectives: This study evaluated the maximum depth and percentage of irrigant penetration into dentinal tubules by passive ultrasonic irrigation (PUI). Materials and Methods: Thirty extracted human teeth were instrumented and divided into three groups. According to final irrigation regimen, 5.25% sodium hypochlorite (Group A, NaOCl), 2% chlorhexidine (Group B, CHX) and saline solution (Group C, control group) were applied with Irrisafe 20 tips (Acteon) and PUI. Irrigant was mixed with 0.1% rhodamine B. Sections at 2 mm, 5 mm, and 8 mm from the apex were examined with confocal laser scanning microscopy (CLSM). The percentage and maximum depth of irrigant penetration were measured. Kruskal-Wallis test and Mann-Whitney test were performed for overall comparison between groups at each level and for pairwise comparison, respectively. Within a group, Wilcoxon test was performed among different levels. p values less than 0.05 were considered significant. Results: In all groups, highest penetration depth and percentage of penetration were observed at the 8 mm level. At 2 mm level, Groups A and B had significantly greater depths and percentages in penetration than Group C (p < 0.05), but there were no significant differences between Groups A and B. At 5 mm level, penetration depths and percentage of penetration was not significantly different among the groups. Conclusions: NaOCl and CHX applied by PUI showed similar depth and percentage of penetration at all evaluated levels.

참고문헌 (Reference)

1 D'Alpino PH, "Use of fluorescent compounds in assessing bonded resin-based restorations: a literature review" 34 : 623-634, 2006

2 Plotino G, "Ultrasonics in endodontics: a review of the literature" 33 : 81-95, 2007

3 Ahmad M, "Ultrasonic debridement of root canals: acoustic cavitation and its relevance" 14 : 486-493, 1988

4 Diaspro A, "Two-photon excitation of fluorescence for three-dimensional optical imaging of biological structures" 55 : 1-8, 2000

5 van der Sluis LW, "The influence of volume, type of irrigant and flushing method on removing artificially placed dentine debris from the apical root canal during passive ultrasonic irrigation" 39 : 472-476, 2006

6 White RR, "The influence of the smeared layer upon dentinal tubule penetration by plastic filling materials" 10 : 558-562, 1984

7 Heling I, "The antimicrobial effect within dentinal tubules of four root canal sealers" 22 : 257-259, 1996

8 Zeltner M, "Temperature changes during ultrasonic irrigation with different inserts and modes of activation" 35 : 573-577, 2009

9 van der Sluis LW, "Study on the influence of refreshment/activation cycles and irrigants on mechanical cleaning efficiency during ultrasonic activation of the irrigant" 36 : 737-740, 2010

10 Ferreira RB, "Sousa-Passive ultrasonic irrigation Neto M. Effectiveness of root canal debris removal using passive ultrasound irrigation with chlorhexidine digluconate or sodium hypochlorite individually or in combination as irrigants" 9 : 68-75, 2008

11 Balguerie E, "Sealer penetration and adaptation in the dentinal tubules: a scanning electron microscopic study" 37 : 1576-1579, 2011

12 Gu LS, "Review of contemporary irrigant agitation techniques and devices" 35 : 791-804, 2009

13 Baca P, "Residual effectiveness of final irrigation regimens on Enteroccus faecalisinfected root canals" 37 : 1121-1123, 2011

14 Shokouhinejad N, "Penetration of Epiphany, Epiphany self-etch, and AH Plus into dentinal tubules: a scanning electron microscopy study" 37 : 1316-1319, 2011

15 van der Sluis LW, "Passive ultrasonic irrigation of the root canal: a review of the literature" 40 : 415-426, 2007

16 Al-Jadaa A, "Necrotic pulp dissolution by passive ultrasonic irrigation in simulated accessory canals: impact of canal location and angulation" 42 : 59-65, 2009

17 유연지, "Multivariate analysis of the cleaning efficacy of different final irrigation techniques in the canal and isthmus of mandibular posterior teeth" 대한치과보존학회 38 (38): 154-159, 2013

18 Love RM, "Invasion of dentinal tubules by oral bacteria" 13 : 171-183, 2002

19 Grande NM, "Interaction between EDTA and sodium hypochlorite: a nuclear magnetic resonance analysis" 32 : 460-464, 2006

20 Leonardo MR, "In vivo antimicrobial activity of 2% chlorhexidine used as root canal irrigating solution" 25 : 167-171, 1999

21 Paragliola R, "Final rinse optimization:influence of different agitation protocols" 36 : 282-285, 2010

22 Bronnec F, "Ex vivo assessment of irrigant penetration and renewal during the final irrigation regimen" 43 : 663-672, 2010

23 De Moor RJ, "Efficacy of ultrasonic versus laseractivated irrigation to remove artificially placed dentin debris plugs" 36 : 1580-1583, 2010

24 de Gregorio C, "Efficacy of different irrigation and activation systems on the penetration of sodium hypochlorite into simulated lateral canals and up to working length: an in vitro study" 36 : 1216-1221, 2010

25 Castagna F, "Effect of passive ultrassonic instrumentation as a final irrigation protocol on debris and smear layer removal-a SEM analysis" 76 : 496-502, 2013

26 Marcilene Coelho Vinhorte, "Effect of passive ultrasonic agitation during final irrigation on cleaning capacity of hybrid instrumentation" 대한치과보존학회 39 (39): 104-108, 2014

27 Vera J, "Effect of maintaining apical patency on irrigant penetration into the apical third of root canals when using passive ultrasonic irrigation: an in vivo study" 37 : 1276-1278, 2011

28 Moon YM, "Effect of laser-activated irrigation of 1320-nanometer Nd:YAG laser on sealer penetration in curved root canals" 38 : 531-535, 2012

29 Moon YM, "Effect of final irrigation regimen on sealer penetration in curved root canals" 36 : 732-736, 2010

30 Kara Tuncer A, "Effect of different final irrigation solutions on dentinal tubule penetration depth and percentage of root canal sealer" 38 : 860-863, 2012

31 Lin LM, "Do procedural errors cause endodontic treatment failure?" d136 : 187-193, 2005

32 Alves FR, "Disinfecting oval-shaped root canals:effectiveness of different supplementary approaches" 37 : 496-501, 2011

33 Zapata RO, "Confocal laser scanning microscopy is appropriate to detect viability of Enterococcus faecalis in infected dentin" 34 : 1198-1201, 2008

34 Bolles JA, "Comparison of Vibringe, EndoActivator, and needle irrigation on sealer penetration in extracted human teeth" 39 : 708-711, 2013

35 Desai P, "Comparative safety of various intracanal irrigation systems" 35 : 545-549, 2009

36 de Vasconcelos BC, "Cleaning ability of chlorhexidine gel and sodium hypochlorite associated or not with EDTA as root canal irrigants: a scanning electron microscopy study" 15 : 387-391, 2007

37 Parmar D, "Bacterial localization and viability assessment in human ex vivo dentinal tubules by fluorescence confocal laser scanning microscopy" 44 : 644-651, 2011

38 Nagayoshi M, "Antimicrobial effect of ozonated water on bacteria invading dentinal tubules" 30 : 778-781, 2004

39 Townsend C, "An in vitro comparison of new irrigation and agitation techniques to ultrasonic agitation in removing bacteria from a simulated root canal" 35 : 1040-1043, 2009

40 Niu W, "A scanning electron microscopic study of dentinal erosion by final irrigation with EDTA and NaOCl solutions" 35 : 934-939, 2002