Protective and Therapeutic Effects of Aloe Vera Gel on Ulcerative Colitis Induced by Acetic Acid in Rats = Protective and Therapeutic Effects of Aloe Vera Gel on Ulcerative Colitis Induced by Acetic Acid in Rats

다국어 초록 (Multilingual Abstract)

Ulcerative colitis (UC) is a chronic intestinal inflammation. Common clinical symptoms are weight loss, diarrhea, ulcers, and inflammation. Aloe vera (AV) has several medicinal properties including antioxidant, anti-inflammatory analgesic, and improvement of gastric and skin ulcers. This study aimed to investigate the protective and therapeutic effects of AV gel on acetic acid-induced UC in rats. UC was induced in 48 rats by injection of 4% acetic acid into the rectum. Protective and treatment groups received treatments 7 days before and after the induction of colitis, respectively. The negative control group, the positive control group, and AV groups received distilled water, sulfasalazine, and 50 and 300 mg/kg of gel extract, respectively. Water and food intake and body weight changes were recorded. The extent of the mucosal ulcers, colon tissue thickening, and mucosal bleeding were scored by the Gerald classification system score (microscopy observations). Slides of tissues were prepared for pathologic assay using the modified Wallace method (macroscopic observations). The results of the macroscopic and microscopic examination showed protective and therapeutic effects of 50 mg/kg dose of AV on acetic acid-induced colitis in rats which reduces the inflammation, ulcers and tissue damage compared with negative control (p < 0.05). There were no significant changes in the amount of water and food intake, body weight changes, and colon weight in protective and treatment groups. Based on the results, AV gel could be used to improve the symptoms of UC, as well as prevent people who are susceptible to the UC.

참고문헌 (Reference)

1 Rieder F, "Wound healing and fibrosis in intestinal disease" 56 : 130-139, 2007

2 Ordás I, "Ulcerative colitis" 380 : 1606-1619, 2012

3 Hekmatpou D, "The effect of Aloe vera clinical trials on prevention and healing of skin wound : a systematic review" 44 : 1-9, 2019

4 Yusuf S, "The effect of Aloe vera A. Berger (Liliaceae) on gastric acid secretion and acute gastric mucosal injury in rats" 93 : 33-37, 2004

5 Niu X, "Protective effect of sanguinarine against acetic acid-induced ulcerative colitis in mice" 267 : 256-265, 2013

6 Al-Rejaie SS, "Protective effect of naringenin on acetic acid-induced ulcerative colitis in rats" 19 : 5633-5644, 2013

7 Branski D, "Prostaglandins in disorders of the intestinal tract : a review" 5 : 853-860, 1986

8 Uhlig HH, "Monogenic diseases associated with intestinal inflammation : implications for the understanding of inflammatory bowel disease" 62 : 1795-1805, 2013

9 Hayat MK, "In vitro antimicrobial activity of Aloe vera L. extracts against pathogenic bacteria and fungi" 14 : 21-27, 2016

10 Morris GP, "Hapten-induced model of chronic inflammation and ulceration in the rat colon" 96 : 795-803, 1989

11 Longstreth GF, "Functional bowel disorders" 130 : 1480-1491, 2006

12 Babitha S, "Fresh fruit juice of Opuntia dillenii Haw. attenuates acetic acid-induced ulcerative colitis in rats" 16 : 431-442, 2019

13 Wallace JL, "Exacerbation of experimental colitis by nonsteroidal anti-inflammatory drugs is not related to elevated leukotriene B4 synthesis" 102 : 18-27, 1992

14 Choudhury D, "Evaluation of anti-inflammatory effect of aqueous extract of Aloe vera in Albino rats" 5 : 2488-2495, 2016

15 Ligumsky M, "Enhanced thromboxane A2 and prostacyclin production by cultured rectal mucosa in ulcerative colitis and its inhibition by steroids and sulfasalazine" 81 : 444-449, 1981

16 Williams KL, "Enhanced survival and mucosal repair after dextran sodium sulfate-induced colitis in transgenic mice that overexpress growth hormone" 120 : 925-937, 2001

17 Zhong YQ, "Effects of sulfasalazine on biopsy mucosal pathologies and histological grading of patients with active ulcerative colitis" 11 : 4435-4438, 2005

18 Yu Z, "Effect of Aloe vera polysaccharides on immunity and antioxidant activities in oral ulcer animal models" 75 : 307-311, 2009

19 Dehury S, "Effect of Aloe vera leaf extract in animal model of ulcerative colitis" 4 : 346-350, 2017

20 Robert A, "Cytoprotection by prostaglandins" 77 : 761-767, 1979

21 Baumgart DC, "Crohn's disease" 380 : 1590-1605, 2012

22 Bush J, "Comparison of Aloe vera and omeprazole in the treatment of equine gastric ulcer syndrome" 50 : 34-40, 2018

23 Haroon SM, "Comparative study of antioxidant activity of flower of Aloe vera and leaf extract of Aloe ferox" 14 : 191-196, 2018

24 Lobos EA, "Chemotactic activity in inflammatory bowel disease. Role of leukotriene B4" 32 : 1380-1388, 1987

25 Gao Y, "Biomedical applications of Aloe vera" 59 : S244-S256, 2019

26 Rajasekaran S, "Beneficial effects of Aloe vera leaf gel extract on lipid profile status in rats with streptozotocin diabetes" 33 : 232-237, 2006

27 Vázquez B, "Antiinflammatory activity of extracts from Aloe vera gel" 55 : 69-75, 1996

28 Langmead L, "Anti-inflammatory effects of Aloe vera gel in human colorectal mucosa in vitro" 19 : 521-527, 2004

29 Bhalsinge RR, "Anti-inflammatory and immunomodulatory activity of ethanol extract of Aloe vera gel" 9 : 832-835, 2018

30 Low D, "Animal models of ulcerative colitis and their application in drug research" 7 : 1341-1357, 2013

31 Goyal N, "Animal models of inflammatory bowel disease : a review" 22 : 219-233, 2014

32 Gao J, "Andrographolide sulfonate ameliorates chronic colitis induced by TNBS in mice via decreasing inflammation and fibrosis" 83 : 106426-, 2020

33 Pilehvar-Soltanahmadi Y, "An overview on application of natural substances incorporated with electrospun nanofibrous scaffolds to development of innovative wound dressings" 18 : 414-427, 2018

34 Arora MK, "Amelioration of diabetes-induced diabetic nephropathy by Aloe vera : implication of oxidative stress and hyperlipidemia" 16 : 227-244, 2019

35 Sharrif MM, "Aloe vera their chemicals composition and applications : a review" 2 : 466-471, 2011

36 Sari Y, "A comparative study of the effects of nigella sativa oil gel and Aloe vera gel on wound healing in diabetic rats" 23 : 2018

37 Puneet Kaur Randhawa, "A Review on Chemical-Induced Inflammatory Bowel Disease Models in Rodents" 대한약리학회 18 (18): 279-288, 2014