Suppression of Cyclooxygenase-2 Expression in Colonic Epithelial Cells by Ilekudinol B Isolated from Weigela subsessilis

다국어 초록 (Multilingual Abstract)

B is one of the flavonoids isolated from Weigela subsessilis (Caprifoliaceae). In the presentstudy, the suppresion effect of ilekudinol B on tumor necrosis factor (TNF)-α-induced cyclooxygenase-2 (COX-prostaglandin E2 (PGE2) secretion was measured by enzyme-linked immunosorbent assay (ELISA). COX-2 andnuclear factor (NF)-κB expresion were determined by Western blot analysis. Ilekudinol B significantly inhibitedTNF-α-induced secretion of IL-8 and prostaglandin E2 (PGE2) from the human colon epithelial cell line HT-29 ina concentration-dependent manner. In addition, ilekudinol B remarkably diminished TNF-α-induced COX-2expression and NF-κB p65 subunit translocation to the nucleus. In conclusion, our results indicate that ilekudinolα-dependent colonic inflamation.KeywordsWeigela subsesilis, ilekudinol B, Caprifoliaceae, cyclooxygenase-2, human colon epithelial cells