저분자 키토산 함유 해양심층수의 알코올대사에 미치는 효과 = The Effect of Deep Sea Water Containing Low Molecular Weight Chitosan on Alcohol Metabolism

국문 초록 (Abstract)

본 연구에서는 저분자 키토산과 해양심층수가 알코올 대사에 미치는 영향을 알아보기 위하여 해양심층수와 저분자 키토산을 함유한 해양심층수를 실험동물에 3주 동안 투여한 후 알코올 대사효소인 ADH, MEOS 및 ALDH의 활성, 혈중 알코올 농도를 측정하였다. 그 결과 간에서 알코올을 일차적으로 아세트알데히드로 산화시키는 효소인 ADH의 활성은 알코올만 투여한 군과 해양심층수 투여군에 비해 저분자 키토산을 함유한 해양심층수를 투여한 군이 27.8~29.9 unit로 증가하였다. 그리고 MEOS 활성도 저분자 키토산 함유한 해양심층수 투여군이 해양심층수에 비해 유의적인 증가를 나타내었다. 숙취 및 세포상해의 원인물질인 아세트알데히드를 분해하는 ALDH도 저분자 키토산을 함유한 해양심층수 (경도 1,500)를 투여한 군에서 13.0 unit로 유의적인 높은 활성을 보였다. 혈중 알코올 함량에서도 해양심층수 단독 투여군보다 저분자 키토산 함유한 해양심층수를 마우스에 투여한 군에서 유의적으로 낮게 나타났다. 이상의 결과에서 보는 바와 같이 저분자 키토산 함유한 해양심층수 (경도 1,500)는 아급성 알코올 중독시 간세포내의 알코올 대사에 관여하는 효소인 ADH, MEOS 및 ALDH의 활성을 증가 시킴으로서 알코올 분해대사를 촉진시켜 혈중 알코올 함량을 낮게 해 주는데 효과가 있음을 알 수 있었다.

다국어 초록 (Multilingual Abstract)

This study was done to investigate the effect of deep sea water (DSW) containing low molecular weight chitosan (LMC) on alcohol-metabolizing enzymes activities in ICR mice. The mice were orally administered 25% ethanol (alcohol group), 25% ethanol and DSW (hardness 0 or 1,500) (DSW group), 25% ethanol and DSW (hardness 0 or 1,500) containing LMC (2.5 mg/ 0.3 mL/day) (DSW containing LMC group) for 3 weeks. Alcohol dehydrogenase (ADH) activity was increased in the DSW (hardness 1,500) containing LMC group compared to the alcohol and DSW group. Also, in the DSW (hardness 1,500) containing LMC group, Microsomal ethanol oxidizing system (MEOS) and aldehyde dehydrogenase (ALDH) activities showed significantly increasing tendency compared to the other groups. The blood alcohol content was lower due to the increased activities of alcohol-metabolizing enzymes (ADH, MEOS, and ALDH) in the DSW (hardness 1,500) containing LMC group. These results suggest that the DSW (hardness 1,500) containing LMC may alleviate ethanol-induced hepatotoxicity by altering alcoholmetabolizing enzymes activities.

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